Penicillium chrysogenum as a fungal factory for feruloyl esterases.

Autor: García-Calvo L; INBIOTEC (Instituto de Biotecnología de León), Avda. Real 1 - Parque Científico de León, 24006, León, Spain.; Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, N-7491, Trondheim, Norway., Rodríguez-Castro R; INBIOTEC (Instituto de Biotecnología de León), Avda. Real 1 - Parque Científico de León, 24006, León, Spain., Ullán RV; INBIOTEC (Instituto de Biotecnología de León), Avda. Real 1 - Parque Científico de León, 24006, León, Spain. rvicu@unileon.es.; mAbxience, Upstream Production, Parque Tecnológico de León, Julia Morros, S/N, Armunia, 24009, León, Spain. rvicu@unileon.es., Albillos SM; Área de Bioquímica Y Biología Molecular, Departamento de Biotecnología Y Ciencia de los Alimentos, Facultad de Ciencias, Universidad de Burgos, 09001, Burgos, Spain., Fernández-Aguado M; INBIOTEC (Instituto de Biotecnología de León), Avda. Real 1 - Parque Científico de León, 24006, León, Spain., Vicente CM; INBIOTEC (Instituto de Biotecnología de León), Avda. Real 1 - Parque Científico de León, 24006, León, Spain.; TBI, Université de Toulouse, CNRS, INRAE, INSA, 31077, Toulouse, France., Degnes KF; Department of Biotechnology and Nanomedicine, SINTEF Industry, Richard Birkelands Vei 3 B, 7034, Trondheim, Norway., Sletta H; Department of Biotechnology and Nanomedicine, SINTEF Industry, Richard Birkelands Vei 3 B, 7034, Trondheim, Norway., Barreiro C; Área de Bioquímica Y Biología Molecular, Departamento de Biología Molecular, Universidad de León, Campus de Vegazana, 24007, León, Spain. c.barreiro@unileon.es.
Jazyk: angličtina
Zdroj: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Feb; Vol. 107 (2-3), pp. 691-717. Date of Electronic Publication: 2023 Jan 03.
DOI: 10.1007/s00253-022-12335-w
Abstrakt: Plant biomass is a promising substrate for biorefinery, as well as a source of bioactive compounds, platform chemicals, and precursors with multiple industrial applications. These applications depend on the hydrolysis of its recalcitrant structure. However, the effective biological degradation of plant cell walls requires several enzymatic groups acting synergistically, and novel enzymes are needed in order to achieve profitable industrial hydrolysis processes. In the present work, a feruloyl esterase (FAE) activity screening of Penicillium spp. strains revealed a promising candidate (Penicillium rubens Wisconsin 54-1255; previously Penicillium chrysogenum), where two FAE-ORFs were identified and subsequently overexpressed. Enzyme extracts were analyzed, confirming the presence of FAE activity in the respective gene products (PrFaeA and PrFaeB). PrFaeB-enriched enzyme extracts were used to determine the FAE activity optima (pH 5.0 and 50-55 °C) and perform proteome analysis by means of MALDI-TOF/TOF mass spectrometry. The studies were completed with the determination of other lignocellulolytic activities, an untargeted metabolite analysis, and upscaled FAE production in stirred tank reactors. The findings described in this work present P. rubens as a promising lignocellulolytic enzyme producer. KEY POINTS: • Two Penicillium rubens ORFs were first confirmed to have feruloyl esterase activity. • Overexpression of the ORFs produced a novel P. rubens strain with improved activity. • The first in-depth proteomic study of a P. rubens lignocellulolytic extract is shown.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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