Complete genome sequence and genome characterization of a novel potyvirus from Lamprocapnos spectabilis.

Autor: Igori D; Department of Biology, School of Mathematics and Natural Sciences, Mongolian National University of Education, Ulaanbaatar, Mongolia.; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea., Shin AY; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea., Kim SE; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea., Choi EK; Jangchun Seed Company, 72 Sideok-ro, Chilgok-gun, Yakmokmyeon, Kyeongsangbuk-do, 39821, Republic of Korea., Hwang US; Jangchun Seed Company, 72 Sideok-ro, Chilgok-gun, Yakmokmyeon, Kyeongsangbuk-do, 39821, Republic of Korea., Kwon SY; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea. sykwon@kribb.re.kr.; Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea. sykwon@kribb.re.kr., Moon JS; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea. jsmoon@kribb.re.kr.; Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea. jsmoon@kribb.re.kr.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2023 Jan 03; Vol. 168 (1), pp. 25. Date of Electronic Publication: 2023 Jan 03.
DOI: 10.1007/s00705-022-05654-w
Abstrakt: The genome of a new potyvirus from a Lamprocapnos spectabilis plant in South Korea was sequenced by high-throughput sequencing and confirmed by Sanger sequencing. The new potyvirus was tentatively named "lamprocapnos virus A" (LaVA); its complete genome contains 9,745 nucleotides, excluding the 3'-terminal poly(A) tail. The LaVA genome structure is similar to that of members of the genus Potyvirus and contains an open reading frame encoding a large putative polyprotein of 3,120 amino acids (aa) with conserved motifs. The complete genome shared 48%-56% nucleotide sequence identity and the polyprotein shared 41%-52% aa sequence identity with those of other potyviruses. These values are below the standard thresholds for potyvirus species demarcation. Phylogenetic analysis based on polyprotein sequences showed that LaVA belongs to the genus Potyvirus. To our knowledge, this is the first report of the complete genome sequence and genome characterization of a potyvirus infecting Lamprocapnos spectabilis.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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