Blueberry Leaf Extract Prevents Lacrimal Hyposecretion in Sjögren's Syndrome-like Model of Non-obese Diabetic Mice.

Autor:	Ogawa K; Institute for Tenure Track Promotion, University of Miyazaki, Miyazaki, Japan; ogawa.kenjirou.u2@cc.miyazaki-u.ac.jp., Urata K; Graduate School of Agriculture, University of Miyazaki, Miyazaki, Japan., Maeda S; Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan., Ohno Y; Department of Pharmacology, Asahi University School of Dentistry, Mizuho, Japan., Satoh K; Department of Pharmacology, Meikai University School of Dentistry, Sakado, Japan., Yamada Y; Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan., Suzuki Y; Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Miyazaki, Japan., Koga Y; Graduate School of Agriculture, University of Miyazaki, Miyazaki, Japan., Sugamoto K; Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Miyazaki, Japan., Kawaguchi M; Department of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan., Kunitake H; Graduate School of Agriculture, University of Miyazaki, Miyazaki, Japan.; Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan., Nishiyama K; Graduate School of Agriculture, University of Miyazaki, Miyazaki, Japan.; Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan., Goto YO; Biolabo Co., Ltd., Kobe, Japan., Nakayama T; Biolabo Co., Ltd., Kobe, Japan., Yamasaki M; Graduate School of Agriculture, University of Miyazaki, Miyazaki, Japan.; Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Jazyk:	angličtina
Zdroj:	In vivo (Athens, Greece) [In Vivo] 2023 Jan-Feb; Vol. 37 (1), pp. 149-162.
DOI:	10.21873/invivo.13064
Abstrakt:	Background/aim: This study evaluated the effect of blueberry leaf hot water extract (BLEx) on Sjögren's syndrome (SS)-like lacrimal hyposecretion in male non-obese diabetic (NOD) mice. Materials and Methods: NOD or BALB/c mice were fed 1% BLEx or control (AIN-93G) for 2 weeks from the age of 4 to 6 weeks. Pilocarpine-induced tear volume was measured using a phenol red-impregnated thread. The lacrimal glands were evaluated histologically by H&E staining. The IL-1β and TNF-α levels in the lacrimal gland tissue were measured by ELISA. The mRNA expression levels of secretion-related proteins were measured by real-time PCR. LC3 I/II and arginase 1 expression levels were measured by western blot. Results: After feeding with BLEx, pilocarpine-induced tear secretion in NOD mice was increased. In contrast, the mRNA expression levels of the cholinergic muscarinic M3 receptor, aquaporin 5, and ion channels related to lacrimal secretion were not changed by BLEx administration. In addition, the protein expression of arginase 1, which was recently reported to be involved in tear hyposecretion in NOD mice, was also not improved by BLEx administration. Although infiltration in the lacrimal gland of NOD mice was not decreased, the levels of TNF-α and the autophagy-related protein LC3 were significantly suppressed by BLEx treatment. Conclusion: BLEx treatment may ameliorate lacrimal hyposecretion in NOD mice by delaying the progression of autoimmune disease by suppressing autophagy in lacrimal glands. (Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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