A colorimetric lateral flow immunoassay based on oriented antibody immobilization for sensitive detection of SARS-CoV-2.

Autor: Lee AS; Graduate School of Biochemistry, Yeungnam University, Gyeongsan 38541, Republic of Korea., Kim SM; Graduate School of Biochemistry, Yeungnam University, Gyeongsan 38541, Republic of Korea., Kim KR; Graduate School of Biochemistry, Yeungnam University, Gyeongsan 38541, Republic of Korea., Park C; Vaccine Bio Research Institute, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul 06591, Republic of Korea., Lee DG; Vaccine Bio Research Institute, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul 06591, Republic of Korea.; Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul 06591, Republic of Korea., Heo HR; Senotherapy-based Metabolic Disease Control Research Center, Yeungnam University, Gyeongsan 38541, Republic of Korea., Cha HJ; Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 37673, Republic of Korea., Kim CS; Graduate School of Biochemistry, Yeungnam University, Gyeongsan 38541, Republic of Korea.; School of Chemistry and Biochemistry, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Jazyk: angličtina
Zdroj: Sensors and actuators. B, Chemical [Sens Actuators B Chem] 2023 Mar 15; Vol. 379, pp. 133245. Date of Electronic Publication: 2022 Dec 26.
DOI: 10.1016/j.snb.2022.133245
Abstrakt: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19). The high human-to-human transmission and rapid evolution of SARS-CoV-2 have resulted in a worldwide pandemic. To contain SARS-CoV-2, it is essential to efficiently control the transmission of the virus through the early diagnosis of infected individuals, including asymptomatic people. Therefore, a rapid and accurate assay is vital for the early diagnosis of SARS-CoV-2 in suspected individuals. In this study, we developed a colorimetric lateral flow immunoassay (LFIA) in which a CBP31-BC linker was used to immobilize antibodies on a cellulose membrane in an oriented manner. The developed LFIA enabled sensitive detection of cultured SARS-CoV-2 in 15 min with a detection limit of 5 × 10 4 copies/mL. The clinical performance of the LFIA for detecting SARS-CoV-2 was evaluated using 19 clinical samples validated by reverse transcription-polymerase chain reaction (RT-PCR). The LFIA detected all the positive and negative samples accurately, corresponding to 100% accuracy. Importantly, patient samples with low viral loads were accurately identified. Thus, the proposed method can provide a useful platform for rapid and accurate point-of-care testing of SARS-CoV-2 in infected individuals to efficiently control the COVID-19 pandemic.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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