Simultaneous determination of L-tryptophan impurities in meat products.

Autor: Lee DH; National Instrumentation Center for Environmental Management, Seoul National University, Seoul, 08826, Republic of Korea., Kim YH; Institute of Biotechnology, CJ CheilJedang, Suwon, Gyeonggi, 16495, Republic of Korea. yanghee.kim@cj.net., Baek M; Institute of Biotechnology, CJ CheilJedang, Suwon, Gyeonggi, 16495, Republic of Korea., Heo IK; Institute of Biotechnology, CJ CheilJedang, Suwon, Gyeonggi, 16495, Republic of Korea., Shin Y; Institute of Biotechnology, CJ CheilJedang, Suwon, Gyeonggi, 16495, Republic of Korea.
Jazyk: angličtina
Zdroj: Amino acids [Amino Acids] 2023 Feb; Vol. 55 (2), pp. 173-182. Date of Electronic Publication: 2023 Jan 02.
DOI: 10.1007/s00726-022-03215-8
Abstrakt: L-tryptophan has been used as a feed additive for swine and poultry and as a nutrient supplement for humans. However, some impurities in L-tryptophan have been reported as causative components of eosinophilia-myalgia syndrome. Therefore, from a safety perspective, it is important to analyze meat samples for these impurities. This study aims to develop an analytical method for the simultaneous detection of L-tryptophan impurities in meat products using LC-MS/MS. Among the various impurities, detection methods for (S)-2-amino-3-(5-hydroxy-1H-indol-3-yl)propanoic acid (5-hydroxytryptophan) (HTP), 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (MTCA), 3a-hydroxy-1,2,3,3a,8,8a-hexahydropyrrolo-[2,3-b]-indole-2-carboxylic acid (PIC), and 1,1'-ethylidenebistryptophan (EBT) and 2-(3-indoylmethyl)-L-tryptophan (IMT) were developed. The developed method allowed simultaneous determination of these four impurities in 5 min. No interferences from the matrix were observed, and the method showed good sensitivity to each analyte. The method detection limit and limit of quantification in meat matrices were below 11.2 and 35.7 μg/kg, respectively.
(© 2022. The Author(s).)
Databáze: MEDLINE
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