Online SFE-SFC-MS/MS colony screening: A high-throughput approach for optimizing (-)-limonene production.

Autor: Takekana M; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan., Yoshida T; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan., Yoshida E; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan; Research Institute for Bioscience Products & Fine Chemicals. Ajinomoto Co., Inc. Kanagawa, Japan., Ono S; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan., Horie S; Shimadzu Corporation, Kyoto, Japan., Vavricka CJ; Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan., Hiratani M; Research Institute for Bioscience Products & Fine Chemicals. Ajinomoto Co., Inc. Kanagawa, Japan., Tsuge K; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan., Ishii J; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan; Engineering Biology Research Center, Kobe University, Kobe, Japan., Hayakawa Y; Shimadzu Corporation, Kyoto, Japan., Kondo A; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan; Engineering Biology Research Center, Kobe University, Kobe, Japan., Hasunuma T; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan; Engineering Biology Research Center, Kobe University, Kobe, Japan. Electronic address: hasunuma@port.kobe-u.ac.jp.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2023 Jan 15; Vol. 1215, pp. 123588. Date of Electronic Publication: 2022 Dec 28.
DOI: 10.1016/j.jchromb.2022.123588
Abstrakt: Conventional analysis of microbial bioproducers requires the extraction of metabolites from liquid cultures, where the culturing steps are time consuming and greatly limit throughput. To break through this barrier, the current study aims to directly evaluate microbial bioproduction colonies by way of supercritical fluid extraction-supercritical fluid chromatography-triple quadrupole mass spectrometry (SFE-SFC-MS/MS). The online SFE-SFC-MS/MS system offers great potential for high-throughput analysis due to automated metabolite extraction without any need for pretreatment. This is the first report of SFE-SFC-MS/MS as a method for direct colony screening, as demonstrated in the high-throughput screening of (-)-limonene bioproducers. Compared with conventional analysis, the SFE-SFC-MS/MS system enables faster and more convenient screening of highly productive strains.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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