Proteomic Assessment of the Murine Mammary Gland Extracellular Matrix.

Autor:	Preston SEJ; Division of Experimental Medicine, McGill University, Montreal, QC, Canada.; Gerald Bronfman Department of Oncology, McGill University, Montreal, QC, Canada.; Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Canada., Richard VR; Segal Cancer Proteomics Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC, Canada.; Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Canada., Del Rincón SV; Division of Experimental Medicine, McGill University, Montreal, QC, Canada.; Gerald Bronfman Department of Oncology, McGill University, Montreal, QC, Canada.; Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Canada., Borchers CH; Division of Experimental Medicine, McGill University, Montreal, QC, Canada. christoph.borchers@mcgill.ca.; Gerald Bronfman Department of Oncology, McGill University, Montreal, QC, Canada. christoph.borchers@mcgill.ca.; Segal Cancer Proteomics Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC, Canada. christoph.borchers@mcgill.ca.; Department of Pathology, Jewish General Hospital, McGill University, Montreal, Canada. christoph.borchers@mcgill.ca.; Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Canada. christoph.borchers@mcgill.ca., Zahedi RP; Manitoba Centre for Proteomics and Systems Biology, Winnipeg, MB, Canada. rene.zahedi@umanitoba.ca.; Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada. rene.zahedi@umanitoba.ca.; Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, Canada. rene.zahedi@umanitoba.ca.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2614, pp. 261-271.
DOI:	10.1007/978-1-0716-2914-7_16
Abstrakt:	The extracellular matrix (ECM) is a molecular scaffold mainly comprising fibrous proteins, glycoproteins, proteoglycans, and polysaccharides. Aside from acting as a structural support, the ECM's composition dictates cell-matrix interactions at the biochemical and biophysical level. In the context of cancer, the ECM is a critical component of the tumor microenvironment (TME) and dysregulation of its deposition and remodelling has been shown to promote tumor onset, progression, and metastasis. Here, we describe a robust protocol for the isolation and subsequent proteomic analysis of the ECM of murine mammary glands, for downstream assays studying the role of the ECM in breast cancer. The protocol yields sufficient protein amounts to enable not only the global quantitation of protein expression but furthermore the enrichment and quantitative analysis of post-translationally modified peptides to study aberrant signalling. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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