Metformin and empagliflozin modulate monoamine oxidase-related oxidative stress and improve vascular function in human mammary arteries.

Autor: Lascu A; Department III Functional Sciences-Pathophysiology, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania.; Centre for Translational Research and Systems Medicine, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania.; Institute of Cardiovascular Diseases Timișoara, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania., Ionică LN; Centre for Translational Research and Systems Medicine, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania.; Doctoral School Medicine-Pharmacy, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania., Buriman DG; Centre for Translational Research and Systems Medicine, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania.; Doctoral School Medicine-Pharmacy, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania., Merce AP; Centre for Translational Research and Systems Medicine, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania.; Institute of Cardiovascular Diseases Timișoara, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania., Deaconu L; Department VII Internal Medicine-Diabetes, Nutrition and Metabolic Diseases, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania., Borza C; Department III Functional Sciences-Pathophysiology, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania.; Centre for Translational Research and Systems Medicine, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania., Crețu OM; Department IX Surgery-Surgical Semiotics I, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania.; Centre for Hepato-Biliary and Pancreatic Surgery, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania., Sturza A; Department III Functional Sciences-Pathophysiology, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania. sturza.adrian@umft.ro.; Centre for Translational Research and Systems Medicine, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania. sturza.adrian@umft.ro., Muntean DM; Department III Functional Sciences-Pathophysiology, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania.; Centre for Translational Research and Systems Medicine, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania., Feier HB; Institute of Cardiovascular Diseases Timișoara, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania.; Department VI Cardiology-Cardiovascular Surgery, 'Victor Babeș' University of Medicine and Pharmacy From Timișoara, Eftimie Murgu Sq. No. 2, 300041, Timișoara, Romania.
Jazyk: angličtina
Zdroj: Molecular and cellular biochemistry [Mol Cell Biochem] 2023 Sep; Vol. 478 (9), pp. 1939-1947. Date of Electronic Publication: 2022 Dec 30.
DOI: 10.1007/s11010-022-04633-8
Abstrakt: Monoamine oxidases (MAOs), mitochondrial enzymes with two isoforms, A and B, have been recently recognized as significant contributors to oxidative stress in the cardiovascular system. The present study was purported to assess the effect of metformin and empagliflozin on MAO expression, oxidative stress and vascular reactivity in internal mammary arteries harvested from overweight patients with coronary heart disease subjected to bypass grafting. Vascular rings were prepared and acutely incubated (12 h) with high glucose (GLUC, 400 mg/dL) or angiotensin II (AII, 100 nM) and metformin (10 µM) and/or empagliflozin (10 µM) and used for the assessment of MAO expression (qRT-PCR and immune histochemistry), reactive oxygen species (ROS, confocal microscopy and spectrophotometry), and vasomotor function (myograph). Ex vivo stimulation with GLUC or AII increased both MAOs expression, ROS production and impaired relaxation to acetylcholine (ACh) of the vascular rings. All effects were alleviated by incubation with each antidiabetic drug; no cumulative effect was obtained when the drugs were applied together. In conclusion, MAO-A and B are upregulated in mammary arteries after acute stimulation with GLUC and AII. Endothelial dysfunction and oxidative stress were alleviated by either metformin or empagliflozin in both stimulated and non-stimulated vascular samples harvested from overweight cardiac patients.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: