Fatty acid composition and metabolic partitioning of α-linolenic acid are contingent on life stage in human CD3 + T lymphocytes.

Autor:	West AL; School of Human Development and Health, Faculty of Medicine, University of Southampton, Southampton, Hampshire, United Kingdom., von Gerichten J; Department of Nutritional Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, United Kingdom., Irvine NA; School of Human Development and Health, Faculty of Medicine, University of Southampton, Southampton, Hampshire, United Kingdom., Miles EA; School of Human Development and Health, Faculty of Medicine, University of Southampton, Southampton, Hampshire, United Kingdom., Lillycrop KA; Centre for Biological Sciences, Faculty of Natural and Environmental Sciences, University of Southampton, Southampton, Hampshire, United Kingdom., Calder PC; School of Human Development and Health, Faculty of Medicine, University of Southampton, Southampton, Hampshire, United Kingdom.; National Institute for Health and Care Research (NIHR) Southampton Biomedical Research Centre, University Hospital Southampton National Health Service (NHS) Foundation Trust and University of Southampton, Southampton, Hampshire, United Kingdom., Fielding BA; Department of Nutritional Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, United Kingdom., Burdge GC; School of Human Development and Health, Faculty of Medicine, University of Southampton, Southampton, Hampshire, United Kingdom.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2022 Dec 13; Vol. 13, pp. 1079642. Date of Electronic Publication: 2022 Dec 13 (Print Publication: 2022).
DOI:	10.3389/fimmu.2022.1079642
Abstrakt:	Introduction: Immune function changes across the life course; the fetal immune system is characterised by tolerance while that of seniors is less able to respond effectively to antigens and is more pro-inflammatory than in younger adults. Lipids are involved centrally in immune function but there is limited information about how T cell lipid metabolism changes during the life course. Methods and Results: We investigated whether life stage alters fatty acid composition, lipid droplet content and α-linolenic acid (18:3ω-3) metabolism in human fetal CD3 + T lymphocytes and in CD3 + T lymphocytes from adults (median 41 years) and seniors (median 70 years). Quiescent fetal T cells had higher saturated (SFA), monounsaturated fatty acid (MUFA), and ω-6 polyunsaturated fatty acid (PUFA) contents than adults or seniors. Activation-induced changes in fatty acid composition differed between life stages. The principal metabolic fates of [ 13 C]18:3ω-3 were constitutive hydroxyoctadecatrienoic acid synthesis and β-oxidation and carbon recycling into SFA and MUFA. These processes declined progressively across the life course. Longer chain ω-3 PUFA synthesis was a relatively minor metabolic fate of 18:3ω-3 at all life stages. Fetal and adult T lymphocytes had similar lipid droplet contents, which were lower than in T cells from seniors. Variation in the lipid droplet content of adult T cells accounted for 62% of the variation in mitogen-induced CD69 expression, but there was no significant relationship in fetal cells or lymphocytes from seniors. Discussion: Together these findings show that fatty acid metabolism in human T lymphocytes changes across the life course in a manner that may facilitate the adaptation of immune function to different life stages. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 West, von Gerichten, Irvine, Miles, Lillycrop, Calder, Fielding and Burdge.)
Databáze:	MEDLINE
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