Combining IP 3 affinity chromatography and bioinformatics reveals a novel protein-IP 3 binding site on Plasmodium falciparum MDR1 transporter.

Autor: Alves E; Life Science Department, Imperial College London, London, United Kingdom., Nakaya H; Department of Clinical and Toxicological Analyses of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.; Computational Systems Biology Laboratory, INOVA, University of Sao Paulo, Sao Paulo, Brazil., Guimarães E; Federal University of Rio Grande do Norte, Pharmacy Department, Health Science Center, Natal, Brazil., Garcia CRS; Department of Clinical and Toxicological Analyses of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Current research in microbial sciences [Curr Res Microb Sci] 2022 Dec 18; Vol. 4, pp. 100179. Date of Electronic Publication: 2022 Dec 18 (Print Publication: 2023).
DOI: 10.1016/j.crmicr.2022.100179
Abstrakt: Intracellular Ca 2+ mobilization induced by second messenger IP 3 controls many cellular events in most of the eukaryotic groups. Despite the increasing evidence of IP 3 -induced Ca 2+ in apicomplexan parasites like  Plasmodium , responsible for malaria infection, no protein with potential function as an IP 3 -receptor has been identified. The use of bioinformatic analyses based on previously known sequences of IP 3 -receptor failed to identify potential IP 3 -receptor candidates in any  Apicomplexa . In this work, we combine the biochemical approach of an IP 3 affinity chromatography column with bioinformatic meta-analyses to identify potential vital membrane proteins that present binding with IP 3 in  Plasmodium falciparum . Our analyses reveal that PF3D7_0523000, a gene that codes a transport protein associated with multidrug resistance as a potential target for IP 3 . This work provides a new insight for probing potential candidates for IP 3 -receptor in  Apicomplexa .
Competing Interests: The authors declare no conflict of financial or commercial interests.
(© 2022 The Authors. Published by Elsevier B.V.)
Databáze: MEDLINE
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