Antibacterial activity of silver nanoparticles functionalized with amikacin applied against multidrug-resistant acinetobacter baumannii.

Autor: Camargo LO; Bionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos/SP, Brazil., Fontoura I; Bionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos/SP, Brazil., Veriato TS; Bionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos/SP, Brazil., Raniero L; Nanosensors Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos/SP, Brazil., Castilho ML; Bionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos/SP, Brazil. Electronic address: mcastilho@univap.br.
Jazyk: angličtina
Zdroj: American journal of infection control [Am J Infect Control] 2023 Aug; Vol. 51 (8), pp. 871-878. Date of Electronic Publication: 2022 Dec 26.
DOI: 10.1016/j.ajic.2022.12.009
Abstrakt: Background: Multidrug-resistant bacteria are one of the world's biggest health problems; therefore, improving the spectrum of action of antibiotics could be necessary to reverse this situation. Amikacin and silver salts have well-known antimicrobial properties. However, both drugs lost their effectiveness against some bacteria, such as Acinetobacter baumannii. This work aims to develop a nanodrug from silver nanoparticles (AgNPs) functionalized with Amikacin against multidrug-resistant Acinetobacter baumannii.
Methods: AgNPs were produced using the bottom-up methodology and functionalized with Amikacin modified by the carbodiimide-based chemistry, forming AgNPs@Amikacin. Susceptibility tests were performed using Amikacin-resistant Acinetobacter baumannii strains to assess the bacteriostatic and bactericidal potential of the developed nanodrug. The clinical strains were induced to form a biofilm, and biomass quantification and the metabolic activity were determined.
Results: The AgNPs have a hydrodynamic diameter of the particles with a bimodal distribution, with a size of 37.84 nm. The FT-IR spectrum of AgNPs@Amikacin exhibits vibrational modes corresponding to Amikacin, confirming the conjugation to AgNPs. Susceptibility testing demonstrated a minimal inhibitory and bactericidal concentration of < 0.5 µg/mL. The AgNPs@Amikacin reduced the biofilm metabolic activity of Acinetobacter baumannii at rates ≥ 50%, characterized by the minimal biofilm inhibition concentrations.
Conclusions: Results demonstrate a promising development of a new nanodrug with lower concentrations, less toxicity, and greater efficacy against multidrug-resistant Acinetobacter baumannii.
(Copyright © 2023 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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