The synergistic effect of using bacteriophages and chitosan nanoparticles against pathogenic bacteria as a novel therapeutic approach.

Autor: Abdelsattar AS; Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, Giza 12578, Egypt. Electronic address: p-abdallah.abdelsattar@zewailcity.edu.eg., Yakoup AY; Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, Giza 12578, Egypt. Electronic address: s-aghapy.yakoup@zewailcity.edu.eg., Khaled Y; Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, Giza 12578, Egypt. Electronic address: s-yousefkhaled@zewailcity.edu.eg., Safwat A; Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, Giza 12578, Egypt., El-Shibiny A; Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, Giza 12578, Egypt; Faculty of Environmental Agricultural Sciences, Arish University, Arish 45511, Egypt. Electronic address: aelshibiny@zewailcity.edu.eg.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Feb 15; Vol. 228, pp. 374-384. Date of Electronic Publication: 2022 Dec 26.
DOI: 10.1016/j.ijbiomac.2022.12.246
Abstrakt: Public health and environmental security are seriously at risk due to the growing contamination of pathogenic microorganisms. Therefore, effective antimicrobials are urgently needed. In our study, the antimicrobial effects of three types of nanoparticles were investigated with phage. The biosynthesis of nanoparticles was confirmed based on the color change and shapes, which tended to be mono-dispersed with a spherical shape with a size range of 20-35 nm for Ag-CS-NPs; 15-30 nm for Phage-CS-NPs (Ph-CS-NPs); and 5-35 nm for Propolis-CS-NPs (Pro-CS-NPs). Nanoparticles displayed peaks between 380-420 nm, 335-380 nm, and below 335 nm for Ag-CS-NPs, Pro-CS-NPs, and Ph-CS NPs, respectively. Throughout the three synthesized nanoparticles, AgCs NPs represented a higher antibacterial effect in combination with phages. It showed MIC against S. sciuri, S. Typhimurium, and P. aeruginosa between 31.2 and 62.2 μg/mL and MBC at 500, 62.5, and 31.2 μg/mL, respectively, while in combination with phages showed MIC at 62.2, 31.2, and 15.6 μg/mL, respectively and MBC at 125, 62.2, and 15.6 μg/mL, respectively. Furthermore, a significant killing efficiency was observed with 16.5-30.1 μg/mL of Ag-CS NPs combined with phages. In conclusion, Ag-CS-NPs with phages present potential bactericidal and inhibitory effects against Gram-positive and Gram-negative bacteria, as well as against the production of biofilms.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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