Immunization with Usutu virus and with a chimeric West Nile virus (WNV) harboring Usutu-E protein protects immunocompetent adult mice against lethal challenges with different WNV lineage 1 and 2 strains.

Autor: Jurisic L; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy; Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy., Malatesta D; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy., Zaccaria G; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy., Di Teodoro G; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy., Bonfini B; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy., Valleriani F; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy., Teodori L; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy., Bencivenga F; Medicina Preventiva e Sanità Pubblica, Direzione Strategica ASL Caserta, Italy., Leone A; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy., Ripà P; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy., D'Innocenzo V; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy., Rossi E; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy., Lorusso A; Istituto Zooprofilattico Sperimentale Teramo (IZS-Te), Campo Boario, Teramo, Italy. Electronic address: a.lorusso@izs.it.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2023 Feb; Vol. 277, pp. 109636. Date of Electronic Publication: 2022 Dec 13.
DOI: 10.1016/j.vetmic.2022.109636
Abstrakt: West Nile virus (WNV) and Usutu virus (USUV), two antigenically related flaviviruses co-circulating in Europe, can cause severe neurological disease in animals and humans. The immune response against USUV and WNV and their immunopathogenesis are still poorly investigated. Here we present results upon sequential infections of adult immunocompetent CD-1 and BALB/c mice primed with two different doses (high dose, HD or low dose, LD) of an USUV isolate and challenged with HD or LD of three different WNV isolates. CD-1 and BALB/c LD USUV-primed mice, regardless of the dose, are largely protected from lethal WNV challenges despite showing no detectable neutralizing antibodies. Furthermore, mice immunized with a chimeric virus harboring the E protein of USUV within the WNV backbone (WNV E-USUV ) are protected against a lethal challenge with WNV. We believe these findings could contribute to understanding the dynamics of the interaction during sequential infection of these two flaviviruses.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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