Non-coding RNAs are correlated to TGF-β receptor type 2 in patients with colorectal cancer.

Autor: Abkenar BR; Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran., Mohammadi A; Shohada Hospital of Tarom, Zanjan University of Medical Sciences, Zanjan, Iran., Amoli HA; Sina Trauma and Surgery Research Center, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran., Soleimani AA; Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran., Korani M; Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran., Mahmoodi H; Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran., Najafi M; Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran.; Microbial Biotechnology Research Center, Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran.
Jazyk: angličtina
Zdroj: The journal of gene medicine [J Gene Med] 2023 Mar; Vol. 25 (3), pp. e3472. Date of Electronic Publication: 2023 Jan 11.
DOI: 10.1002/jgm.3472
Abstrakt: Background: Multiple molecular expression alterations, particularly in non-coding RNAs, play fundamental roles in the regulations of cellular processes and may relate to the occurrence and progression of colorectal cancer (CRC). In the present study, we investigated the associations between TGFBR2, miR20a-5p and long non-coding RNA (lncRNA) LAMTOR5-AS1 in CRC patients.
Methods: Colorectal cancer and adjacent normal tissue samples (n = 34) were prepared from CRC patients. The associations between TGFBR2, miR20a-5p and lncRNA LAMTOR5-AS1 were predicted using bioinformatics tools. The expression levels of TGFBR2, miR20a-5p and lncRNA LAMTOR5-AS1 were measured using a quantitative real-time polymerase chain reaction technique. The TGFBR2 protein values were measured by western blotting. The clinical importance of lncRNA LAMTOR5-AS1 was assessed using receiver operating characteristic curve.
Results: The up-regulated levels of TGFBR2 (p = 0.02), TGFBR2 protein (p = 0.008) and lncRNA LAMTOR5-AS1 (p = 0.02) were significantly observed in CRC tissues compared to the adjacent normal tissues. The miR20a-5p expression level (p = 0.009) was downregulated in CRC tissues. In addition, the miR20a-5p expression level was inversely correlated to the TGFBR2 gene (r 2  = 0.88, p < 0.0001), protein (r 2  = 0.95, p < 0.0001) and lncRNA LAMTOR5-AS1 gene (r 2  = 0.93, p < 0.0001) expression levels. Based on the area under curve, the increase of lncRNA LAMTOR5-AS1 expression level with a sensitivity of 64.52% and specificity of 65.52% was considered in CRC patients.
Conclusions: We propose that miR20a-5p is inversely related to long non-coding RNA (lncRNA) LAMTOR5-AS, such that it may be involved in the regulation of TGFBR2 expression level in CRC patients.
(© 2022 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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