Virus-like particles containing a prefusion-stabilized F protein induce a balanced immune response and confer protection against respiratory syncytial virus infection in mice.

Autor:	Luo J; State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China., Qin H; State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China., Lei L; State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China., Lou W; State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China., Li R; State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China., Pan Z; State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2022 Dec 12; Vol. 13, pp. 1054005. Date of Electronic Publication: 2022 Dec 12 (Print Publication: 2022).
DOI:	10.3389/fimmu.2022.1054005
Abstrakt:	Respiratory syncytial virus (RSV) is a serious respiratory pathogen in infants and young children worldwide. Currently, no licensed RSV vaccines are available. In this study, we explored stable prefusion conformation virus-like particles (Pre-F VLPs) as RSV vaccine candidates. RSV fusion (F) protein mutants were constructed to form stabilized Pre-F or postfusion (Post-F) configurations. VLPs containing Pre-F or Post-F protein were generated using a recombinant baculovirus (rBV)-insect cell expression system. The assembly and immunological properties of Pre-F or Post-F VLPs were investigated. Pre-F and Post-F VLPs contained antigenic sites Ø and I of pre- and postfusion conformations, respectively. Compared with Post-F VLPs, immunization with Pre-F VLPs elicited upregulation of IFN-γ, IL-2 and IL-10 and downregulation of IL-4 and IL-5 cytokine production in mice. A high percentage of CD25 + Foxp3 + cells or a low percentage of IL-17A-producing cells among CD4 + T cells was observed in the lungs of mice vaccinated with Pre-F VLPs. Importantly, immunization with Pre-F VLPs induced a high level of RSV neutralizing antibody and a balanced immune response, which protected mice against RSV infection without evidence of immunopathology. Our results suggested that Pre-F VLPs generated from rBV-insect cells represent promising RSV vaccine candidates. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Luo, Qin, Lei, Lou, Li and Pan.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu