Label-free DNA detection using silver nanoprism decorated silicon nanoparticles: Effect of silicon nanoparticle size and doping levels.

Autor: Daoudi K; Centre for Advanced Materials Research, Research Institute of Sciences and Engineering, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates; Department of Applied Physics and Astronomy, College of Sciences, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates. Electronic address: kdaoudi@sharjah.ac.ae., Columbus S; Centre for Advanced Materials Research, Research Institute of Sciences and Engineering, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates., Falcão BP; CICECO, Department of Physics, University of Aveiro, 3810-193 Aveiro, Portugal; Department of Physics and I3N, University of Aveiro, 3810-193 Aveiro, Portugal., Pereira RN; Department of Physics and I3N, University of Aveiro, 3810-193 Aveiro, Portugal., Peripolli SB; CICECO, Department of Materials and Ceramic Engineering, University of Aveiro, 3810-193 Aveiro, Portugal., Ramachandran K; Centre for Advanced Materials Research, Research Institute of Sciences and Engineering, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates., Hadj Kacem H; Department of Applied Biology, College of Sciences, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates., Allagui A; Centre for Advanced Materials Research, Research Institute of Sciences and Engineering, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates; Department of Sustainable and Renewable Energy Engineering, College of Engineering, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates., Gaidi M; Centre for Advanced Materials Research, Research Institute of Sciences and Engineering, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates; Department of Applied Physics and Astronomy, College of Sciences, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates; Laboratoire de Photovoltaïque, Centre de Recherches et des Technologies de l'Energie, Technopole de Borj-Cédria, Hammam-Lif 2050, Tunisia.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2023 Apr 05; Vol. 290, pp. 122262. Date of Electronic Publication: 2022 Dec 21.
DOI: 10.1016/j.saa.2022.122262
Abstrakt: In the present work, we have fabricated silver nanoprism (AgNPrs)/silicon nanoparticle (SiNPs) hybrid arrays for highly sensitive detection of biomolecules via surface-enhanced Raman spectroscopy (SERS) technique. SiNPs having 7 to 37 nm in size and with phosphorous doping varying from 1 × 10 19 to 1 × 10 20 cm -3 were synthesized in nonthermal plasma synthesis. SiNPs were further immobilized on glass substrates using spin-coating, followed by deposition of AgNPrs using the drop-casting method. SERS studies showed that AgNPrs/SiNPs hybrid arrays exhibit substantial amplification of fingerprint bands of rhodamine 6G (R6G) compared to bare silicon as the reference. Raman signal intensity was found to be dependent on the size of SiNPs, with the largest nanoparticles exhibiting the highest SERS enhancement. In addition, an increase in phosphorous doping concentration was found to reduce R6G peak intensities. AgNPrs/SiNPs hybrid arrays showed excellent stability over time and high spot-to-spot reproducibility as well. Moreover, hybrid arrays enabled DNA detection through intense vibrational modes of human genomic DNA, with a lower detection limit of 1.5 pg/µL; indicating that AgNPrs/SiNPs sensors can serve as a reliable and cost-effective biosensing platform for rapid and label-free analysis of biomolecules.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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