High-frequency synthetic apomixis in hybrid rice.

Autor: Vernet A; CIRAD, AGAP institute, Avenue Agropolis, 34398, Montpellier, France.; University of Montpellier, CIRAD-INRAE-Institut Agro-University of Montpellier, Montpellier, France., Meynard D; CIRAD, AGAP institute, Avenue Agropolis, 34398, Montpellier, France.; University of Montpellier, CIRAD-INRAE-Institut Agro-University of Montpellier, Montpellier, France., Lian Q; Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, D-50829, Cologne, Germany., Mieulet D; CIRAD, AGAP institute, Avenue Agropolis, 34398, Montpellier, France.; University of Montpellier, CIRAD-INRAE-Institut Agro-University of Montpellier, Montpellier, France.; DIADE, University of Montpellier- IRD-CIRAD, Montpellier, France., Gibert O; CIRAD, AGAP institute, Avenue Agropolis, 34398, Montpellier, France.; University of Montpellier, CIRAD-INRAE-Institut Agro-University of Montpellier, Montpellier, France.; CIRAD, UMR Qualisud, Rue Jean-François Breton, 34398, Montpellier, France.; University of Montpellier, Avignon University, CIRAD, Institut Agro, Reunion University, Montpellier, France., Bissah M; CSIR, Plant Genetic Resources Research Institute, P. O. Box 7, Bunso, Ghana., Rivallan R; CIRAD, AGAP institute, Avenue Agropolis, 34398, Montpellier, France.; University of Montpellier, CIRAD-INRAE-Institut Agro-University of Montpellier, Montpellier, France., Autran D; DIADE, University of Montpellier- IRD-CIRAD, Montpellier, France., Leblanc O; DIADE, University of Montpellier- IRD-CIRAD, Montpellier, France., Meunier AC; CIRAD, AGAP institute, Avenue Agropolis, 34398, Montpellier, France.; University of Montpellier, CIRAD-INRAE-Institut Agro-University of Montpellier, Montpellier, France., Frouin J; CIRAD, AGAP institute, Avenue Agropolis, 34398, Montpellier, France.; University of Montpellier, CIRAD-INRAE-Institut Agro-University of Montpellier, Montpellier, France., Taillebois J; CIRAD, AGAP institute, Avenue Agropolis, 34398, Montpellier, France., Shankle K; Dept. of Plant Biology, University of California, 1 Shields Ave, Davis, CA, 95616, USA., Khanday I; Dept. of Plant Sciences, University of California, 1 Shields Ave, Davis, CA, 95616, USA. khanday@ucdavis.edu.; Innovative Genomics Institute, University of California, 2151 Berkeley Way, Berkeley, CA, 94704, USA. khanday@ucdavis.edu., Mercier R; Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, D-50829, Cologne, Germany. mercier@mpipz.mpg.de., Sundaresan V; Dept. of Plant Biology, University of California, 1 Shields Ave, Davis, CA, 95616, USA. sundar@ucdavis.edu.; Dept. of Plant Sciences, University of California, 1 Shields Ave, Davis, CA, 95616, USA. sundar@ucdavis.edu.; Innovative Genomics Institute, University of California, 2151 Berkeley Way, Berkeley, CA, 94704, USA. sundar@ucdavis.edu., Guiderdoni E; CIRAD, AGAP institute, Avenue Agropolis, 34398, Montpellier, France. emmanuel.guiderdoni@cirad.fr.; University of Montpellier, CIRAD-INRAE-Institut Agro-University of Montpellier, Montpellier, France. emmanuel.guiderdoni@cirad.fr.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Dec 27; Vol. 13 (1), pp. 7963. Date of Electronic Publication: 2022 Dec 27.
DOI: 10.1038/s41467-022-35679-3
Abstrakt: Introducing asexual reproduction through seeds - apomixis - into crop species could revolutionize agriculture by allowing F1 hybrids with enhanced yield and stability to be clonally propagated. Engineering synthetic apomixis has proven feasible in inbred rice through the inactivation of three genes (MiMe), which results in the conversion of meiosis into mitosis in a line ectopically expressing the BABYBOOM1 (BBM1) parthenogenetic trigger in egg cells. However, only 10-30% of the seeds are clonal. Here, we show that synthetic apomixis can be achieved in an F1 hybrid of rice by inducing MiMe mutations and egg cell expression of BBM1 in a single step. We generate hybrid plants that produce more than 95% of clonal seeds across multiple generations. Clonal apomictic plants maintain the phenotype of the F1 hybrid along successive generations. Our results demonstrate that there is no barrier to almost fully penetrant synthetic apomixis in an important crop species, rendering it compatible with use in agriculture.
(© 2022. The Author(s).)
Databáze: MEDLINE
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