[Effects of electroacupuncture on the expression of β-amyloid and autophagy-related proteins in hippocampal cells of mice with Alzheimer's disease].

Autor:	Tan YX; College of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China., Zhang Y; College of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China., Gao YS; College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China., Pei YN; Jincheng People's Hospital of Shanxi Province, Jincheng 048000, Shanxi Province., Yang XK; College of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China., Li CL; College of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China., Xue WG; College of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
Jazyk:	čínština
Zdroj:	Zhen ci yan jiu = Acupuncture research [Zhen Ci Yan Jiu] 2022 Dec 25; Vol. 47 (12), pp. 1048-53.
DOI:	10.13702/j.1000-0607.20211232
Abstrakt:	Objective: To investigate the effect of electroacupuncture (EA) on the expression of β-amyloid (Aβ) and autophagy-related proteins in hippocampal cells of Alzheimer's disease (AD) model mice, so as to explore its underlying mechanisms. Methods: Eighteen male APP/PS1 transgenic mice (6 months old) were randomly divided into model and EA groups, with 9 mice in each group. Nine male C57BL/6 wild-type mice of the same age were chosen as the normal group. Mice in the EA group were treated with acupuncture on "Baihui" (GV20) and EA (1 Hz/50 Hz, 1 mA) on bilateral "Yongquan" (KI1), once every other day, 20 min each time for a total of 21 times. After the interventions, the spatial learning and memory ability were observed by Morris water maze test. The autophagy-related pathological changes in hippocampus were observed by transmission electron microscopy. The expressions of microtublue associated protein 1 light chain 3 (LC3) and Aβ in hippocampus were observed by immunofluorescence and the expression levels of LC3 and p62 proteins were detected by Western blot. Results: Compared with the normal group, the escape latency was prolonged ( P <0.01), the residence time in the original quadrant platform was shor-tened ( P <0.05), the positive expressions of LC3 and Aβ, the expression levels of LC3Ⅱ and p62 proteins, and the ratio of LC3Ⅱ/LC3Ⅰ proteins in hippocampus were increased ( P <0.01, P <0.05) in the model group. Compared with the model group, the escape latency was shortened ( P <0.05), the residence time in the original quadrant platform was prolonged ( P <0.05), the positive expressions of LC3 and Aβ, the expression levels of LC3Ⅱ and p62 proteins, and the ratio of LC3Ⅱ/LC3Ⅰ proteins in hippocampus were decreased ( P <0.05) in the EA group. The transmission electron microscopy showed that the structure of neurons was normal in the normal group, a large number of autolysosomes and autophagosomes existed in hip-pocampal nerve cells in the model group, and only a small number of autophagosomes were observed in the EA group. Conclusion: EA can reduce the expression levels of autophagy-related proteins LC3 and p62 in APP/PS1 transgenic mice, improve the hip-pocampal autophagy state, reduce intracellular Aβ aggregation, and thus improve the learning and memory ability.
Databáze:	MEDLINE
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