High resolution 16S rRNA gene Next Generation Sequencing study of brain areas associated with Alzheimer's and Parkinson's disease.

Autor:	Emery DC; Bristol Dental School, Bristol, United Kingdom., Davies M; Bristol Dental School, Bristol, United Kingdom., Cerajewska TL; Bristol Dental School, Bristol, United Kingdom., Taylor J; Bristol Dental School, Bristol, United Kingdom., Hazell M; Translational Health Sciences, Learning and Research, Bristol Medical School, Southmead Hospital, Bristol, United Kingdom., Paterson A; School of Biological Sciences, University of Bristol Genomics Facility, Bristol, United Kingdom., Allen-Birt SJ; Translational Health Sciences, Learning and Research, Bristol Medical School, Southmead Hospital, Bristol, United Kingdom., West NX; Bristol Dental School, Bristol, United Kingdom.
Jazyk:	angličtina
Zdroj:	Frontiers in aging neuroscience [Front Aging Neurosci] 2022 Dec 09; Vol. 14, pp. 1026260. Date of Electronic Publication: 2022 Dec 09 (Print Publication: 2022).
DOI:	10.3389/fnagi.2022.1026260
Abstrakt:	Introduction: Alzheimer's (AD) and Parkinson's disease (PD) are neurodegenerative conditions characterized by incremental deposition of β-amyloid (Aβ) and α-synuclein in AD and PD brain, respectively, in relatively conserved patterns. Both are associated with neuroinflammation, with a proposed microbial component for disease initiation and/or progression. Notably, Aβ and α-synuclein have been shown to possess antimicrobial properties. There is evidence for bacterial presence within the brain, including the oral pathobiont Porphyromonas gingivalis , with cognitive impairment and brain pathology being linked to periodontal (gum) disease and gut dysbiosis. Methods: Here, we use high resolution 16S rRNA PCR-based Next Generation Sequencing (16SNGS) to characterize bacterial composition in brain areas associated with the early, intermediate and late-stage of the diseases. Results and Discussion: This study reveals the widespread presence of bacteria in areas of the brain associated with AD and PD pathology, with distinctly different bacterial profiles in blood and brain. Brain area profiles were overall somewhat similar, predominantly oral, with some bacteria subgingival and oronasal in origin, and relatively comparable profiles in AD and PD brain. However, brain areas associated with early disease development, such as the locus coeruleus, were substantially different in bacterial DNA content compared to areas affected later in disease etiology. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Emery, Davies, Cerajewska, Taylor, Hazell, Paterson, Allen-Birt and West.)
Databáze:	MEDLINE
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