Children gut microbiota exhibits a different composition and metabolic profile after in vitro exposure to Clostridioides difficile and increases its sporulation.

Autor:	Horvat S; Department of Microbiology, Faculty of Medicine, University of Maribor, Maribor, Slovenia., Mahnic A; Department of Microbiology, Faculty of Medicine, University of Maribor, Maribor, Slovenia.; Centre for Medical Microbiology, National Laboratory of Health, Environment and Food, Maribor, Slovenia., Makuc D; Slovenian NMR Centre, National Institute of Chemistry, Ljubljana, Slovenia., Pečnik K; Slovenian NMR Centre, National Institute of Chemistry, Ljubljana, Slovenia., Plavec J; Slovenian NMR Centre, National Institute of Chemistry, Ljubljana, Slovenia., Rupnik M; Department of Microbiology, Faculty of Medicine, University of Maribor, Maribor, Slovenia.; Centre for Medical Microbiology, National Laboratory of Health, Environment and Food, Maribor, Slovenia.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2022 Dec 09; Vol. 13, pp. 1042526. Date of Electronic Publication: 2022 Dec 09 (Print Publication: 2022).
DOI:	10.3389/fmicb.2022.1042526
Abstrakt:	Clostridioides difficile (Clostridium difficile) infection (CDI) is one of the main public health concerns in adults, while children under 2 years of age are often colonized asymptomatically. In both adults and children, CDI is strongly associated with disturbances in gut microbiota. In this study, an in-vitro model of children gut microbiota was challenged with vegetative cells or a conditioned media of six different toxigenic C. difficile strains belonging to the ribotypes 027, 078, and 176. In the presence of C. difficile or conditioned medium the children gut microbiota diversity decreased and all main phyla ( Bacteroidetes, Firmicutes , and Proteobacteria ) were affected. The NMR metabolic spectra divided C. difficile exposed children gut microbiota into three clusters. The grouping correlated with nine metabolites (short chain fatty acids, ethanol, phenolic acids and tyramine). All strains were able to grow in the presence of children gut microbiota and showed a high sporulation rate of up to 57%. This high sporulation rate in combination with high asymptomatic carriage in children could contribute to the understanding of the reported role of children in C. difficile transmissions. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Horvat, Mahnic, Makuc, Pečnik, Plavec and Rupnik.)
Databáze:	MEDLINE
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