Genotype classification of Moraxella bovis using MALDI-TOF MS profiles.

Autor:	Olson HG; School of Veterinary Medicine and Biomedical Sciences, Institute for Agriculture and Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United States., Loy JD; School of Veterinary Medicine and Biomedical Sciences, Institute for Agriculture and Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United States., Clawson ML; United States Department of Agricultural, Agricultural Research Service, United States Meat Animal Research Center, Clay Center, NE, United States., Wynn EL; United States Department of Agricultural, Agricultural Research Service, United States Meat Animal Research Center, Clay Center, NE, United States., Hille MM; School of Veterinary Medicine and Biomedical Sciences, Institute for Agriculture and Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2022 Dec 08; Vol. 13, pp. 1057621. Date of Electronic Publication: 2022 Dec 08 (Print Publication: 2022).
DOI:	10.3389/fmicb.2022.1057621
Abstrakt:	Moraxella bovis ( M. bovis ) is regarded as a causative agent of infectious bovine keratoconjunctivitis (IBK), the most common ocular disease of cattle. Recently, whole genome sequencing identified the presence of two distinct genotypes within M. bovis that differ in chromosome content, potential virulence factors, as well as prophage and plasmid profiles. It is unclear if the genotypes equally associate with IBK or if one is more likely to be isolated from IBK lesions. We utilized 39 strains of M. bovis that had previously undergone whole genome sequencing and genotype classification to determine the utility of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) to accurately genotype M. bovis strains. We successfully developed two biomarker models that accurately classified strains according to genotype with an overall accuracy of 85.8-100% depending upon the model and sample preparation method used. These models provide a practical tool to enable studies of genotype associations with disease, allow for epidemiological studies at the sub-species level, and can be used to enhance disease prevention strategies. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Olson, Loy, Clawson, Wynn and Hille.)
Databáze:	MEDLINE
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