Presumptive bacteriological diagnosis of spondylodiscitis in infants less than 4 years by detecting K. kingae DNA in their oropharynx: Data from a preliminar two centers study.

Autor:	Chargui M; Unité D'orthopédie Pédiatrique et de Traumatologie Infantile, Service de Chirurgie Pédiatrique, Hôpitaux Universitaires de Genève, Genève, Switzerland., Krzysztofiak A; Infectious Diseases Unit, Academic Department of Pediatrics, Bambino Gesù Children's Hospital (IRCCS), Rome, Italy., Bernaschi P; Microbiology Unit, Bambino Gesù Children's Hospital (IRCCS), Rome, Italy., De Marco G; Unité D'orthopédie Pédiatrique et de Traumatologie Infantile, Service de Chirurgie Pédiatrique, Hôpitaux Universitaires de Genève, Genève, Switzerland., Coulin B; Unité D'orthopédie Pédiatrique et de Traumatologie Infantile, Service de Chirurgie Pédiatrique, Hôpitaux Universitaires de Genève, Genève, Switzerland., Steiger C; Unité D'orthopédie Pédiatrique et de Traumatologie Infantile, Service de Chirurgie Pédiatrique, Hôpitaux Universitaires de Genève, Genève, Switzerland., Dayer R; Unité D'orthopédie Pédiatrique et de Traumatologie Infantile, Service de Chirurgie Pédiatrique, Hôpitaux Universitaires de Genève, Genève, Switzerland., Ceroni D; Unité D'orthopédie Pédiatrique et de Traumatologie Infantile, Service de Chirurgie Pédiatrique, Hôpitaux Universitaires de Genève, Genève, Switzerland.
Jazyk:	angličtina
Zdroj:	Frontiers in pediatrics [Front Pediatr] 2022 Dec 09; Vol. 10, pp. 1046254. Date of Electronic Publication: 2022 Dec 09 (Print Publication: 2022).
DOI:	10.3389/fped.2022.1046254
Abstrakt:	Background and Objectives: Most cases of spondylodiscitis in children aged between 6 and 48 months old could be caused primarily by K. kingae. The present prospective study aimed to determine whether an innovative and indirect diagnosis approach - based on detection of K. kingae DNA in the oropharynx of children with suspected spondylodiscitis - provides sufficient evidence that this microorganism is responsible for the infection. Methods: We prospectively analysed infants admitted for spondylodiscitis, considering above all the results of PCR realized in oropharyngeal swabs and in blood samples. Results: Four of the 29 performed K. kingae-specific real-time PCR assay in blood were positive (13.8%), whereas 28 of the 32 K. kingae-specific real-time PCR assay realized on throat swabs were positive (87.5%). Conclusions: This study demonstrates that performing oropharyngeal swab PCR is able to detect K. kingae in almost 90% of the toddlers with confirmed spondylodiscitis. That provides strong arguments for the hypothesis that K. kingae should be considered as the main aetiological pathogen to suspect in children between 6 and 48 months old with spondylodiscitis. Finally, it seems to us reasonable that oropharyngeal swab may become an early decision-making tool for the indirect identification of K. kingae in spondylodiscitis. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© 2022 Chargui, Krzysztofiak, Bernaschi, De Marco, Coulin, Steiger, Dayer and Ceroni.)
Databáze:	MEDLINE
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