Growth dynamics and transcriptional responses of a Red Sea Prochlorococcus strain to varying temperatures.

Autor: Labban A; Marine Science, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia.; Environmental Science and Engineering, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia., Shibl AA; Genetic Heritage Group, Biology Program, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates.; Public Health Research Center, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates., Calleja ML; Climate Geochemistry Department, Max Plank Institute for Chemistry, Mainz, Germany., Hong PY; Environmental Science and Engineering, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia., Morán XAG; Marine Science, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia.; Centro Oceanográfico de Gijón/Xixón, Instituto Español de Oceanografía (IEO-CSIC), Gijón/Xixón, Spain.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2023 May; Vol. 25 (5), pp. 1007-1021. Date of Electronic Publication: 2023 Jan 21.
DOI: 10.1111/1462-2920.16326
Abstrakt: Prochlorococcus play a crucial role in the ocean's biogeochemical cycling, but it remains controversial how they will respond to global warming. Here we assessed the response to temperature (22-30°C) of the growth dynamics and gene expression profiles of a Red Sea Prochlorococcus strain (RSP50) in a non-axenic culture. Both the specific growth rate (0.55-0.80 day -1 ) and cell size (0.04-0.07 μm 3 ) of Prochlorococcus increased significantly with temperature. The primary production released extracellularly ranged from 20% to 34%, with humic-like fluorescent compounds increasing up to fivefold as Prochlorococcus reached its maximum abundance. At 30°C, genes involved in carbon fixation such as CsoS2 and CsoS3 and photosynthetic electron transport including PTOX were downregulated, suggesting a cellular homeostasis and energy saving mechanism response. In contrast, PTOX was found upregulated at 22°C and 24°C. Similar results were found for transaldolase, related to carbon metabolism, and citrate synthase, an important enzyme in the TCA cycle. Our data suggest that in spite of the currently warm temperatures of the Red Sea, Prochlorococcus can modulate its gene expression profiles to permit growth at temperatures lower than its optimum temperature (28°C) but is unable to cope with temperatures exceeding 30°C.
(© 2022 Applied Microbiology International and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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