Melatonin decreases IRF-3 protein expression in the gastrocnemius muscle, reduces IL-1β and LPS plasma concentrations, and improves the lipid profile in rats with apical periodontitis fed on a high-fat diet.

Autor: Dos Santos RM; School of Dentistry, Araçatuba Department of Basic Sciences, São Paulo State University (UNESP), R. José Bonifácio, 1193, Araçatuba, 16015-050, Brazil. rodrigoms13@hotmail.com.; Programa de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF), Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil. rodrigoms13@hotmail.com., Belardi BE; School of Dentistry, Araçatuba Department of Basic Sciences, São Paulo State University (UNESP), R. José Bonifácio, 1193, Araçatuba, 16015-050, Brazil.; Programa de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF), Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Tsosura TVS; School of Dentistry, Araçatuba Department of Basic Sciences, São Paulo State University (UNESP), R. José Bonifácio, 1193, Araçatuba, 16015-050, Brazil.; Programa de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF), Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Chiba FY; School of Dentistry, Araçatuba Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Mattera MSLC; School of Dentistry, Araçatuba Department of Basic Sciences, São Paulo State University (UNESP), R. José Bonifácio, 1193, Araçatuba, 16015-050, Brazil.; Programa de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF), Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Machado NES; School of Dentistry, Araçatuba Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Cantiga-Silva C; School of Dentistry, Araçatuba Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Carvalho NR; School of Dentistry, Araçatuba Department of Basic Sciences, São Paulo State University (UNESP), R. José Bonifácio, 1193, Araçatuba, 16015-050, Brazil.; Programa de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF), Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Bravo LT; School of Dentistry, Araçatuba Department of Basic Sciences, São Paulo State University (UNESP), R. José Bonifácio, 1193, Araçatuba, 16015-050, Brazil.; Programa de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF), Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Nobumoto ACTY; School of Dentistry, Araçatuba Department of Basic Sciences, São Paulo State University (UNESP), R. José Bonifácio, 1193, Araçatuba, 16015-050, Brazil.; Programa de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF), Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Oliveira SHP; School of Dentistry, Araçatuba Department of Basic Sciences, São Paulo State University (UNESP), R. José Bonifácio, 1193, Araçatuba, 16015-050, Brazil.; Programa de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF), Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Cintra LTA; School of Dentistry, Araçatuba Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Matsushita DH; School of Dentistry, Araçatuba Department of Basic Sciences, São Paulo State University (UNESP), R. José Bonifácio, 1193, Araçatuba, 16015-050, Brazil.; Programa de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF), Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil.
Jazyk: angličtina
Zdroj: Odontology [Odontology] 2023 Jul; Vol. 111 (3), pp. 687-696. Date of Electronic Publication: 2022 Dec 25.
DOI: 10.1007/s10266-022-00782-w
Abstrakt: To evaluate the effects of melatonin (MEL) on the expression of toll-like receptor-4 (TLR4); myeloid differentiation primary response protein-88 (MyD88); TIR-domain-containing adapter-inducing interferon-β (TRIF); IFN regulatory-factor-3 (IRF-3); nuclear factor kappa-B (NF-κB); plasma concentrations of interleukin-1β (IL-1β) and lipopolysaccharide (LPS); and lipid profile of rats with apical periodontitis (AP) fed on a high-fat diet (HFD). Eighty 60-day-old rats were divided into eight groups: control, AP, HFD, HFDAP, CNMEL, APMEL, HFDMEL and HFDAPMEL. HFD groups were fed on a HFD for 107 days. On day 7, experimental AP was induced in the AP groups, and after 70 days, MEL (5 mg/kg) was administered to the MEL groups for 30 days. Plasma concentrations of LPS and IL-1β were analyzed using enzyme-linked immunosorbent assay, and the lipid profile was analyzed using biochemical tests. The expression of proteins involved in the TLR4 pathway (TLR4, MyD88, TRIF, IRF-3 and NF-κB) in the gastrocnemius muscle (GM) was evaluated using western blotting and qRT-PCR. Treatment with MEL decreased IRF-3 protein expression in GM and IL-1β plasma concentration in the APMEL and HFDMEL groups. Reduction in LPS plasma concentration was reported only in the HFDMEL group. Additionally, a decrease in LDL and an increase in HDL were observed in the HFDMEL and HFDAPMEL groups. Treatment with MEL exhibited anti-inflammatory and anti-hyperlipidemic effects attributed to HFD and AP by reducing the plasma concentrations of IL-1β and LPS in addition to reducing IRF-3 protein expression in the GM, which is associated with the production of inflammatory cytokines.
(© 2022. The Author(s), under exclusive licence to The Society of The Nippon Dental University.)
Databáze: MEDLINE
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