Effect of chronic sleep deprivation on acrosomal integrity and functional parameters of murine sperm.

Autor: Adami LNG; Disciplina de Urologia, Setor de Reprodução Humana, Departamento de Cirurgia, Universidade Federal de São Paulo, São Paulo, Brazil., Fernandes GL; Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil., Carvalho RC; Disciplina de Urologia, Setor de Reprodução Humana, Departamento de Cirurgia, Universidade Federal de São Paulo, São Paulo, Brazil., Okada FK; Disciplina de Urologia, Setor de Reprodução Humana, Departamento de Cirurgia, Universidade Federal de São Paulo, São Paulo, Brazil; Laboratório de Biologia do Desenvolvimento, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, Brazil. Electronic address: fatima.okada@unifesp.br., Tufik S; Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil., Andersen ML; Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil., Bertolla RP; Disciplina de Urologia, Setor de Reprodução Humana, Departamento de Cirurgia, Universidade Federal de São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: F&S science [F S Sci] 2023 Feb; Vol. 4 (1), pp. 11-20. Date of Electronic Publication: 2022 Dec 22.
DOI: 10.1016/j.xfss.2022.12.002
Abstrakt: Objective: To evaluate the effect of chronic sleep deprivation on sperm function quality in mice.
Design: Experimental study.
Setting: Not applicable.
Animals: Spermatozoa from twenty-four 10-week-old C57BL/6J male mice.
Intervention(s): The sleep deprivation group underwent gentle handling for 6 hours for 5 consecutive days. The mice in the sleep recovery group were allowed to sleep during the 24-hour period after the sleep deprivation protocol.
Main Outcome Measure(s): After euthanasia, the spermatozoa were collected for analysis. Sperm motility was evaluated using computer-assisted sperm analyzer. Intracellular superoxide anion (O 2 - ) activity, acrosome integrity, mitochondrial activity, and DNA fragmentation assays were conducted afterward.
Result(s): Sleep deprivation and sleep recovery groups presented a lower percentage of spermatozoa with an intact acrosome, compared with the respective control groups. Regarding DNA fragmentation, a decreased proportion of spermatozoa with Comet I class intact DNA was observed in the sleep recovery group, compared with the recovery control group. Beat cross frequency was increased in the sleep recovery group.
Conclusion(s): Sleep deprivation can reduce sperm quality, impairing acrosome integrity. Sleep recovery decreased DNA integrity and increased beat cross frequency.
(Copyright © 2022. Published by Elsevier Inc.)
Databáze: MEDLINE
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