The transcription factor Zic4 promotes tentacle formation and prevents epithelial transdifferentiation in Hydra .

Autor: Vogg MC; Department of Genetics and Evolution, Institute of Genetics and Genomics (iGE3), Faculty of Sciences, University of Geneva, 30 Quai Ernest Ansermet, Geneva 4 1211, Switzerland., Ferenc J; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel 4058, Switzerland.; University of Basel, Petersplatz 1, Basel 4001, Switzerland., Buzgariu WC; Department of Genetics and Evolution, Institute of Genetics and Genomics (iGE3), Faculty of Sciences, University of Geneva, 30 Quai Ernest Ansermet, Geneva 4 1211, Switzerland., Perruchoud C; Department of Genetics and Evolution, Institute of Genetics and Genomics (iGE3), Faculty of Sciences, University of Geneva, 30 Quai Ernest Ansermet, Geneva 4 1211, Switzerland., Sanchez PGL; Department of Genetics and Evolution, Institute of Genetics and Genomics (iGE3), Faculty of Sciences, University of Geneva, 30 Quai Ernest Ansermet, Geneva 4 1211, Switzerland., Beccari L; Institut NeuroMyoGène, CNRS UMR 5310, INSERM U1217, University Claude Bernard Lyon 1, Lyon, France., Nuninger C; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel 4058, Switzerland.; University of Basel, Petersplatz 1, Basel 4001, Switzerland., Le Cras Y; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel 4058, Switzerland., Delucinge-Vivier C; iGE3 Genomics Platform, University of Geneva, 1 Rue Michel-Servet, Geneva 4 1211, Switzerland., Papasaikas P; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel 4058, Switzerland.; SIB Swiss Institute of Bioinformatics, Basel 4058, Switzerland., Vincent S; Laboratoire de Biologie et Modélisation de la Cellule, Ecole Normale Supérieure de Lyon, CNRS, UMR 5239, Inserm, U1293, Université Claude Bernard Lyon 1, 46 allée d'Italie, Lyon F-69364, France., Galliot B; Department of Genetics and Evolution, Institute of Genetics and Genomics (iGE3), Faculty of Sciences, University of Geneva, 30 Quai Ernest Ansermet, Geneva 4 1211, Switzerland., Tsiairis CD; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel 4058, Switzerland.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2022 Dec 23; Vol. 8 (51), pp. eabo0694. Date of Electronic Publication: 2022 Dec 23.
DOI: 10.1126/sciadv.abo0694
Abstrakt: The molecular mechanisms that maintain cellular identities and prevent dedifferentiation or transdifferentiation remain mysterious. However, both processes are transiently used during animal regeneration. Therefore, organisms that regenerate their organs, appendages, or even their whole body offer a fruitful paradigm to investigate the regulation of cell fate stability. Here, we used Hydra as a model system and show that Zic4, whose expression is controlled by Wnt3/β-catenin signaling and the Sp5 transcription factor, plays a key role in tentacle formation and tentacle maintenance. Reducing Zic4 expression suffices to induce transdifferentiation of tentacle epithelial cells into foot epithelial cells. This switch requires the reentry of tentacle battery cells into the cell cycle without cell division and is accompanied by degeneration of nematocytes embedded in these cells. These results indicate that maintenance of cell fate by a Wnt-controlled mechanism is a key process both during homeostasis and during regeneration.
Databáze: MEDLINE
Externí odkaz: