Detection of lisdexamfetamine and its metabolite d-amphetamine in urine and gastric contents collected from a cadaver at forensic autopsy.

Autor: Torimitsu S; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo,, Bunkyo-Ku, Tokyo, 113-0033, Japan. torimitsu@m.u-tokyo.ac.jp.; Department of Legal Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku,, Chiba-Shi, Chiba, 260-8670, Japan. torimitsu@m.u-tokyo.ac.jp., Saka K; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo,, Bunkyo-Ku, Tokyo, 113-0033, Japan., Noritake K; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo,, Bunkyo-Ku, Tokyo, 113-0033, Japan., Namera A; Department of Forensic Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Kasumi 1-2-3, Minami-Ku, Hiroshima, 734-8553, Japan., Makino Y; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo,, Bunkyo-Ku, Tokyo, 113-0033, Japan.; Department of Legal Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku,, Chiba-Shi, Chiba, 260-8670, Japan., Yamaguchi R; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo,, Bunkyo-Ku, Tokyo, 113-0033, Japan.; Department of Legal Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku,, Chiba-Shi, Chiba, 260-8670, Japan., Iwase H; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo,, Bunkyo-Ku, Tokyo, 113-0033, Japan.; Department of Legal Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku,, Chiba-Shi, Chiba, 260-8670, Japan.
Jazyk: angličtina
Zdroj: Forensic toxicology [Forensic Toxicol] 2023 Jul; Vol. 41 (2), pp. 309-317. Date of Electronic Publication: 2022 Dec 23.
DOI: 10.1007/s11419-022-00654-6
Abstrakt: Purpose: Lisdexamfetamine (LDX), which is used for the treatment of attention-deficit/hyperactivity disorder and narcolepsy, is composed of L-lysine attached to dextroamphetamine (d-amphetamine). In this article, we report a forensic autopsy case in which prescription drugs were unknown at autopsy. While amphetamine was detected, methamphetamine could not be detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in any of samples collected. Thus, we aimed to quantify LDX concentrations in autopsy samples and to prove that the amphetamine detected in this case was due to metabolized LDX.
Methods: Femoral vein blood, cardiac whole blood, urine, and gastric content samples were taken at autopsy for toxicological analysis. Qualitative and quantitative analyses were performed using LC-MS/MS. In addition, optical isomer separation for the amphetamine detected was conducted. The stability of LDX in whole blood and urine was also examined at three different temperatures.
Results: The concentrations of LDX were < 4.00, 30.9, and 4.42 ng/mL in whole blood, urine, and gastric content samples, respectively. The concentrations of amphetamine were 329, 510, 2970, and 915 ng/mL in femoral vein blood, heart whole blood, urine, and gastric contents, respectively. The amphetamine detected in this case was identified to be only d-amphetamine by optical isomer separation. The d-amphetamine detected was considered to be derived from LDX. Stability experiments revealed that LDX in whole blood decreased at ambient temperature.
Conclusions: The results in the present case report may be useful in interpreting whether or not the amphetamine detected in a cadaver is a metabolite of LDX.
(© 2022. The Author(s).)
Databáze: MEDLINE
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