Mitochondrial H2S donor AP39 induces stomatal closure by modulating guard cell mitochondrial activity.

Autor:	Pantaleno R; Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata 7600, Argentina., Scuffi D; Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata 7600, Argentina., Costa A; Department of Biosciences, University of Milan, Milan 20133, Italy., Welchen E; Facultad de Bioquímica y Ciencias Biológicas, Instituto de Agrobiotecnología del Litoral (CONICET-UNL). Cátedra de Biología Celular y Molecular, Universidad Nacional del Litoral, Santa Fe 3000, Argentina., Torregrossa R; University of Exeter Medical School, University of Exeter, Exeter, UK., Whiteman M; University of Exeter Medical School, University of Exeter, Exeter, UK., García-Mata C; Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata 7600, Argentina.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2023 Mar 17; Vol. 191 (3), pp. 2001-2011.
DOI:	10.1093/plphys/kiac591
Abstrakt:	Hydrogen sulfide (H2S) is a gaseous signaling molecule involved in numerous physiological processes in plants, including gas exchange with the environment through the regulation of stomatal pore width. Guard cells (GCs) are pairs of specialized epidermal cells that delimit stomatal pores and have a higher mitochondrial density and metabolic activity than their neighboring cells. However, there is no clear evidence on the role of mitochondrial activity in stomatal closure induction. In this work, we showed that the mitochondrial-targeted H2S donor AP39 induces stomatal closure in a dose-dependent manner. Experiments using inhibitors of the mitochondrial electron transport chain (mETC) or insertional mutants in cytochrome c (CYTc) indicated that the activity of mitochondrial CYTc and/or complex IV are required for AP39-dependent stomatal closure. By using fluorescent probes and genetically encoded biosensors we reported that AP39 hyperpolarized the mitochondrial inner potential (Δψm) and increased cytosolic ATP, cytosolic hydrogen peroxide levels, and oxidation of the glutathione pool in GCs. These findings showed that mitochondrial-targeted H2S donors induce stomatal closure, modulate guard cell mETC activity, the cytosolic energetic and oxidative status, pointing to an interplay between mitochondrial H2S, mitochondrial activity, and stomatal closure. Competing Interests: Conflict of interests. M.W and R.T. have intellectual property (patents awarded) on mitochondrial and other targeted H2S donors and MW is CSO of MitoRx Therapeutics. (© American Society of Plant Biologists 2022. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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