Rabies Virus Populations in Humans and Mice Show Minor Inter-Host Variability within Various Central Nervous System Regions and Peripheral Tissues.

Autor: Embregts CWE; Department of Viroscience, Erasmus Medical Centre, 3015 GD Rotterdam, The Netherlands., Farag EABA; Ministry of Public Health, Doha P.O. Box 42, Qatar., Bansal D; Ministry of Public Health, Doha P.O. Box 42, Qatar., Boter M; Department of Viroscience, Erasmus Medical Centre, 3015 GD Rotterdam, The Netherlands., van der Linden A; Department of Viroscience, Erasmus Medical Centre, 3015 GD Rotterdam, The Netherlands., Vaes VP; Department of Viroscience, Erasmus Medical Centre, 3015 GD Rotterdam, The Netherlands., van Middelkoop-van den Berg I; Department of Viroscience, Erasmus Medical Centre, 3015 GD Rotterdam, The Netherlands., IJpelaar J; Department of Viroscience, Erasmus Medical Centre, 3015 GD Rotterdam, The Netherlands., Ziglam H; Hamad Medical Corporation, Doha P.O. Box 3050, Qatar., Coyle PV; Hamad Medical Corporation, Doha P.O. Box 3050, Qatar., Ibrahim I; Hamad Medical Corporation, Doha P.O. Box 3050, Qatar.; Biomedical Research Centre, Qatar University, Doha P.O. Box 2713, Qatar., Mohran KA; Department of Animal Resources, Ministry of Municipality, Doha P.O. Box 35081, Qatar.; Biotechnology Departments ERC, Animal Health Research Institute, Dokki 12611, Egypt., Alrajhi MMS; Department of Animal Resources, Ministry of Municipality, Doha P.O. Box 35081, Qatar., Islam MM; Department of Animal Resources, Ministry of Municipality, Doha P.O. Box 35081, Qatar., Abdeen R; Department of Animal Resources, Ministry of Municipality, Doha P.O. Box 35081, Qatar., Al-Zeyara AA; Department of Animal Resources, Ministry of Municipality, Doha P.O. Box 35081, Qatar., Younis NM; Department of Animal Resources, Ministry of Municipality, Doha P.O. Box 35081, Qatar., Al-Romaihi HE; Ministry of Public Health, Doha P.O. Box 42, Qatar., AlThani MHJ; Ministry of Public Health, Doha P.O. Box 42, Qatar., Sikkema RS; Department of Viroscience, Erasmus Medical Centre, 3015 GD Rotterdam, The Netherlands., Koopmans MPG; Department of Viroscience, Erasmus Medical Centre, 3015 GD Rotterdam, The Netherlands., Oude Munnink BB; Department of Viroscience, Erasmus Medical Centre, 3015 GD Rotterdam, The Netherlands., GeurtsvanKessel CH; Department of Viroscience, Erasmus Medical Centre, 3015 GD Rotterdam, The Netherlands.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2022 Nov 28; Vol. 14 (12). Date of Electronic Publication: 2022 Nov 28.
DOI: 10.3390/v14122661
Abstrakt: Rabies virus (RABV) has a broad host range and infects multiple cell types throughout the infection cycle. Next-generation sequencing (NGS) and minor variant analysis are powerful tools for studying virus populations within specific hosts and tissues, leading to novel insights into the mechanisms of host-switching and key factors for infecting specific cell types. In this study we investigated RABV populations and minor variants in both original (non-passaged) samples and in vitro-passaged isolates of various CNS regions (hippocampus, medulla oblongata and spinal cord) of a fatal human rabies case, and of multiple CNS and non-CNS tissues of experimentally infected mice. No differences in virus populations were detected between the human CNS regions, and only one non-synonymous single nucleotide polymorphism (SNP) was detected in the fifth in vitro passage of virus isolated from the spinal cord. However, the appearance of this SNP shows the importance of sequencing newly passaged virus stocks before further use. Similarly, we did not detect apparent differences in virus populations isolated from different CNS and non-CNS tissues of experimentally infected mice. Sequencing of viruses obtained from pharyngeal swab and salivary gland proved difficult, and we propose methods for improving sampling.
Databáze: MEDLINE
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