Coxiella burnetii in Dogs and Cats from Portugal: Serological and Molecular Analysis.

Autor:	Anastácio S; Vasco da Gama Research Centre (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, Avenida José R. Sousa Fernandes 197 Lordemão, 3020-210 Coimbra, Portugal.; Center of Neurosciences and Cell Biology, Health Science Campus, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal.; Faculty of Pharmacy, University of Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal., Anjos S; Vasco da Gama Research Centre (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, Avenida José R. Sousa Fernandes 197 Lordemão, 3020-210 Coimbra, Portugal.; Baixo Vouga Veterinary Hospital (HVBV), EN 1, 355, 3750-742 Águeda, Portugal., Neves S; Vasco da Gama Research Centre (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, Avenida José R. Sousa Fernandes 197 Lordemão, 3020-210 Coimbra, Portugal.; University Veterinary Hospital of Coimbra (HVUC), Lordemão, 3020-210 Coimbra, Portugal., Neves T; Vasco da Gama Research Centre (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, Avenida José R. Sousa Fernandes 197 Lordemão, 3020-210 Coimbra, Portugal.; University Veterinary Hospital of Coimbra (HVUC), Lordemão, 3020-210 Coimbra, Portugal., Esteves P; University Veterinary Hospital of Coimbra (HVUC), Lordemão, 3020-210 Coimbra, Portugal., Craveiro H; Vasco da Gama Research Centre (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, Avenida José R. Sousa Fernandes 197 Lordemão, 3020-210 Coimbra, Portugal.; Baixo Vouga Veterinary Hospital (HVBV), EN 1, 355, 3750-742 Águeda, Portugal., Madeira B; Animalstaff, R. Quinta da Várzea, Santa Clara, 3030-092 Coimbra, Portugal., Pires MDA; Animal and Veterinary Research Centre (CECAV), University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal.; Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal.; Associate Laboratory for Animal and Veterinary Sciences-AL4AnimalS, 1300-477 Lisbon, Portugal., Sousa S; Vasco da Gama Research Centre (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, Avenida José R. Sousa Fernandes 197 Lordemão, 3020-210 Coimbra, Portugal., da Silva G; Center of Neurosciences and Cell Biology, Health Science Campus, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal.; Faculty of Pharmacy, University of Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal., Vilhena H; Vasco da Gama Research Centre (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, Avenida José R. Sousa Fernandes 197 Lordemão, 3020-210 Coimbra, Portugal.; University Veterinary Hospital of Coimbra (HVUC), Lordemão, 3020-210 Coimbra, Portugal.; Animal and Veterinary Research Centre (CECAV), University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal.; Associate Laboratory for Animal and Veterinary Sciences-AL4AnimalS, 1300-477 Lisbon, Portugal.
Jazyk:	angličtina
Zdroj:	Pathogens (Basel, Switzerland) [Pathogens] 2022 Dec 13; Vol. 11 (12). Date of Electronic Publication: 2022 Dec 13.
DOI:	10.3390/pathogens11121525
Abstrakt:	Dogs and cats are potential sources of infection for some zoonotic diseases such as Q fever, caused by Coxiella burnetii, a multiple host pathogen. Q fever outbreaks in dogs and cats have been related with parturition and abortion events, and ticks have a potential role in the transmission of this pathogen. This study aimed to screen for C. burnetii in dogs and cats, and in ticks collected from infested animals. An observational descriptive study was conducted in Portugal at two time points nine years apart, 2012 and 2021. Sera obtained from dogs and cats (total n = 294) were tested for C. burnetii antibodies using a commercial ELISA adapted for multi-species detection. C. burnetii DNA was screened by qPCR assay targeting IS1111 in uterine samples and in ticks. A decrease in the exposure to C. burnetii was observed in cats from 17.2% (95% CI: 5.8−35.8%) in 2012 to 0.0% in 2021, and in dogs from 12.6% (95% CI: 7.7−19.0%) in 2012 to 1.7% (95% CI: 0.3−9.1%) in 2021 (p < 0.05). Overall, and despite differences in the samples, rural habitat seems to favour the exposure to C. burnetii. The DNA of C. burnetii was not detected in ticks. The low seropositivity observed in 2021 and the absence of C. burnetii DNA in the tested samples, suggest that dogs and cats from Portugal are not often exposed to the pathogen. Nevertheless, the monitoring of C. burnetii infection in companion animals is an important tool to prevent human outbreaks, considering the zoonotic potential for owners and veterinarians contacting infected animals, mainly dogs and cats from rural areas which often come into contact with livestock.
Databáze:	MEDLINE
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