| Autor: |
Averin AS; Institute of Theoretical and Experimental Biophysics, the Russian Academy of Sciences, Pushchino 142290, Russia., Konakov MV; Institute of Theoretical and Experimental Biophysics, the Russian Academy of Sciences, Pushchino 142290, Russia., Pimenov OY; Institute of Theoretical and Experimental Biophysics, the Russian Academy of Sciences, Pushchino 142290, Russia., Galimova MH; Institute of Theoretical and Experimental Biophysics, the Russian Academy of Sciences, Pushchino 142290, Russia., Berezhnov AV; Institute of Cell Biophysics, the Russian Academy of Sciences, Pushchino 142290, Russia., Nenov MN; Institute of Theoretical and Experimental Biophysics, the Russian Academy of Sciences, Pushchino 142290, Russia., Dynnik VV; Institute of Theoretical and Experimental Biophysics, the Russian Academy of Sciences, Pushchino 142290, Russia. |
| Jazyk: |
angličtina |
| Zdroj: |
Membranes [Membranes (Basel)] 2022 Dec 07; Vol. 12 (12). Date of Electronic Publication: 2022 Dec 07. |
| DOI: |
10.3390/membranes12121239 |
| Abstrakt: |
Various models, including stem cells derived and isolated cardiomyocytes with overexpressed channels, are utilized to analyze the functional interplay of diverse ion currents involved in cardiac automaticity and excitation-contraction coupling control. Here, we used β-NAD and ammonia, known hyperpolarizing and depolarizing agents, respectively, and applied inhibitory analysis to reveal the interplay of several ion channels implicated in rat papillary muscle contractility control. We demonstrated that: 4 mM β-NAD, having no strong impact on resting membrane potential (RMP) and action potential duration (APD90) of ventricular cardiomyocytes, evoked significant suppression of isometric force (F) of paced papillary muscle. Reactive blue 2 restored F to control values, suggesting the involvement of P2Y-receptor-dependent signaling in β-NAD effects. Meantime, 5 mM NH 4 Cl did not show any effect on F of papillary muscle but resulted in significant RMP depolarization, APD90 shortening, and a rightward shift of I-V relationship for total steady state currents in cardiomyocytes. Paradoxically, NH 4 Cl, being added after β-NAD and having no effect on RMP, APD, and I-V curve, recovered F to the control values, indicating β-NAD/ammonia antagonism. Blocking of HCN, Kir2.x, and L-type calcium channels, Ca 2+ -activated K + channels (SK, IK, and BK), or NCX exchanger reverse mode prevented this effect, indicating consistent cooperation of all currents mediated by these channels and NCX. We suggest that the activation of Kir2.x and HCN channels by extracellular K + , that creates positive and negative feedback, and known ammonia and K + resemblance, may provide conditions required for the activation of all the chain of channels involved in the interplay. Here, we present a mechanistic model describing an interplay of channels and second messengers, which may explain discovered antagonism of β-NAD and ammonia on rat papillary muscle contractile activity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
|
Nepřihlášeným uživatelům se plný text nezobrazuje |
K zobrazení výsledku je třeba se přihlásit.
|
