| Autor: |
Yildirim F; Department of Pathology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, Istanbul 34500, Turkey., Sudagidan M; Scientific and Technology Application and Research Center, Mehmet Akif Ersoy University, Burdur 15030, Turkey., Aydin A; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, Istanbul 34500, Turkey., Akyazi I; Department of Physiology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, Istanbul 34500, Turkey., Bayrakal GM; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, Istanbul 34500, Turkey., Yavuz O; Scientific and Technology Application and Research Center, Mehmet Akif Ersoy University, Burdur 15030, Turkey., Gurel A; Department of Pathology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, Istanbul 34500, Turkey. |
| Abstrakt: |
Toxin-producing Staphylococcus aureus strains posing a potential risk for public health have long been a topic of scientific research. Effects of Panton-Valentine leukocidin (PVL) on tissue destruction mechanisms and activities of inflammatory cells were presented in animal models of pneumonia and skin infections induced by PVL-producing S. aureus strains. This study aimed to demonstrate the in vivo pathogenicity of PVL-producing S. aureus strains isolated from some foodstuffs, which can be a potential risk to public health. PVL-positive methicillin-susceptible S. aureus (MSSA) strains M1 and YF1B-b isolated from different foodstuffs and a PVL-positive MSSA strain HT480 (positive control) were administered to New Zealand rabbits. Blood samples were harvested three and six hours after the intratracheal inoculation. Lung tissue samples were collected for gross and microscopic exams and immunohistochemical (IHC) demonstration of IL-6, IL8, IL-10, and TNF-α expressions. Serum cytokine levels were also measured by ELISA. The strains isolated from lung tissue samples were confirmed by pulsed-field gel electrophoresis. The development of acute necrotising pneumonia and a significant elevation in IL-6, IL-8, IL-10, and TNF-α expressions demonstrated the significance of foodborne PVL-positive MSSA strains in public health for the first time. |
