Metformin Acutely Mitigates Oxidative Stress in Human Atrial Tissue: A Pilot Study in Overweight Non-Diabetic Cardiac Patients.

Autor: Lascu A; Department III Functional Sciences-Pathophysiology, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania.; Centre for Translational Research and Systems Medicine, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania.; Institute for Cardiovascular Diseases of Timișoara, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, G. Adam Str., No. 13A, 300310 Timișoara, Romania., Ionică LN; Centre for Translational Research and Systems Medicine, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania.; Doctoral School Medicine-Pharmacy, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania., Merce AP; Centre for Translational Research and Systems Medicine, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania.; Institute for Cardiovascular Diseases of Timișoara, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, G. Adam Str., No. 13A, 300310 Timișoara, Romania., Dănilă MD; Department III Functional Sciences-Pathophysiology, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania.; Centre for Translational Research and Systems Medicine, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania., Petrescu L; Centre for Translational Research and Systems Medicine, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania.; Doctoral School Medicine-Pharmacy, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania., Sturza A; Department III Functional Sciences-Pathophysiology, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania.; Centre for Translational Research and Systems Medicine, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania., Muntean DM; Department III Functional Sciences-Pathophysiology, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania.; Centre for Translational Research and Systems Medicine, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania., Streian CG; Institute for Cardiovascular Diseases of Timișoara, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, G. Adam Str., No. 13A, 300310 Timișoara, Romania.; Department VI Cardiology-Cardiovascular Surgery, 'Victor Babeş' University of Medicine and Pharmacy of Timișoara, E. Murgu Sq., No. 2, 300041 Timişoara, Romania.; Advanced Research Center of the Institute for Cardiovascular Diseases, 'Victor Babeş' University of Medicine and Pharmacy Timisoara, E. Murgu Square No. 2, 300041 Timişoara, Romania.
Jazyk: angličtina
Zdroj: Life (Basel, Switzerland) [Life (Basel)] 2022 Dec 08; Vol. 12 (12). Date of Electronic Publication: 2022 Dec 08.
DOI: 10.3390/life12122058
Abstrakt: Metformin, the first-line drug in type 2 diabetes mellitus, elicits cardiovascular protection also in obese patients via pleiotropic effects, among which the anti-oxidant is one of the most investigated. The aim of the present study was to assess whether metformin can acutely mitigate oxidative stress in atrial tissue harvested from overweight non-diabetic patients. Right atrial appendage samples were harvested during open-heart surgery and used for the evaluation of reactive oxygen species (ROS) production by means of confocal microscopy (superoxide anion) and spectrophotometry (hydrogen peroxide). Experiments were performed after acute incubation with metformin (10 µM) in the presence vs. absence of angiotensin II (AII, 100 nM), lipopolysaccharide (LPS, 1 μg/mL), and high glucose (Gluc, 400 mg/dL). Stimulation with AII, LPS, and high Gluc increased ROS production. The magnitude of oxidative stress correlated with several echocardiographic parameters. Metformin applied in the lowest therapeutic concentration (10 µM) was able to decrease ROS generation in stimulated but also non-stimulated atrial samples. In conclusion, in a pilot group of overweight non-diabetic cardiac patients, acute incubation with metformin at a clinically relevant dose alleviated oxidative stress both in basal conditions and conditions that mimicked the activation of the renin-angiotensin-aldosterone system, acute inflammation, and uncontrolled hyperglycemia.
Databáze: MEDLINE
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