Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant-Insect Interactions.

Autor: Oberemok VV; Department of Molecular Genetics and Biotechnologies, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Crimea.; Laboratory of Entomology and Phytopathology, Dendrology and Landscape Architecture, Nikita Botanical Gardens-National Scientific Centre of the Russian Academy of Sciences, Yalta 298648, Crimea., Useinov RZ; Department of Molecular Genetics and Biotechnologies, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Crimea., Skorokhod OA; Department of Life Sciences and Systems Biology, University of Turin, 10124 Turin, Italy., Gal'chinsky NV; Department of Molecular Genetics and Biotechnologies, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Crimea., Novikov IA; Department of Molecular Genetics and Biotechnologies, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Crimea., Makalish TP; S.I. Georgievsky Medical Academy, V.I. Vernadsky Crimean Federal University, Simferopol 295015, Crimea., Yatskova EV; Laboratory of Entomology and Phytopathology, Dendrology and Landscape Architecture, Nikita Botanical Gardens-National Scientific Centre of the Russian Academy of Sciences, Yalta 298648, Crimea., Sharmagiy AK; Laboratory of Entomology and Phytopathology, Dendrology and Landscape Architecture, Nikita Botanical Gardens-National Scientific Centre of the Russian Academy of Sciences, Yalta 298648, Crimea., Golovkin IO; S.I. Georgievsky Medical Academy, V.I. Vernadsky Crimean Federal University, Simferopol 295015, Crimea., Gninenko YI; All-Russian Research Institute for Silviculture and Mechanization of Forestry, Pushkino 141200, Russia., Puzanova YV; Department of Molecular Genetics and Biotechnologies, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Crimea., Andreeva OA; Department of Molecular Genetics and Biotechnologies, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Crimea., Alieva EE; Department of Molecular Genetics and Biotechnologies, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Crimea., Eken E; Department of Molecular Genetics and Biotechnologies, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Crimea., Laikova KV; S.I. Georgievsky Medical Academy, V.I. Vernadsky Crimean Federal University, Simferopol 295015, Crimea., Plugatar YV; Department of Natural Ecosystems, Nikita Botanical Garden-National Scientific Centre of the Russian Academy of Sciences, Yalta 298648, Crimea.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 Dec 10; Vol. 23 (24). Date of Electronic Publication: 2022 Dec 10.
DOI: 10.3390/ijms232415681
Abstrakt: Insects vastly outnumber us in terms of species and total biomass, and are among the most efficient and voracious consumers of plants on the planet. As a result, to preserve crops, one of the primary tasks in agriculture has always been the need to control and reduce the number of insect pests. The current use of chemical insecticides leads to the accumulation of xenobiotics in ecosystems and a decreased number of species in those ecosystems, including insects. Sustainable development of human society is impossible without useful insects, so the control of insect pests must be effective and selective at the same time. In this article, we show for the first time a natural way to regulate the number of insect pests based on the use of extracellular double-stranded DNA secreted by the plant Pittosporum tobira . Using a principle similar to one found in nature, we show that the topical application of artificially synthesized short antisense oligonucleotide insecticides (olinscides, DNA insecticides) is an effective and selective way to control the insect Coccus hesperidum . Using contact oligonucleotide insecticide Coccus-11 at a concentration of 100 ng/μL on C. hesperidum larvae resulted in a mortality of 95.59 ± 1.63% within 12 days. Green oligonucleotide insecticides, created by nature and later discovered by humans, demonstrate a new method to control insect pests that is beneficial and safe for macromolecular insect pest management.
Databáze: MEDLINE
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