| Autor: |
Ponomareva AL; Il'ichev Pacific Oceanological Institute, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia., Eskova AI; Il'ichev Pacific Oceanological Institute, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia., Shakirov RB; Il'ichev Pacific Oceanological Institute, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia., Syrbu NS; Il'ichev Pacific Oceanological Institute, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia., Legkodimov AA; Il'ichev Pacific Oceanological Institute, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia., Grigorov RA; Il'ichev Pacific Oceanological Institute, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia. |
| Abstrakt: |
The bioindication of oil and gas fields is a field of geomicrobiology that is mainly devoted to the detection of hydrocarbon-oxidizing microbial indicator species or functional genes in total DNA. However, it appears promising to use the physiological properties of microorganisms detection deposit type of hydrocarbons, in particular their ability to oxidize hydrocarbons under aerobic and anaerobic conditions. In this study, the most promising approach in this area was the method used for assessing the anaerobic degradation of hydrocarbons. When comparing molecular genetics and cultured methods of bioindication, it can be concluded that molecular biomarkers of functional genes for the anaerobic destruction of hydrocarbons ( masD ) make it possible to separate areas with traditional and gas-hydrate types of deposits. Using cultured methods, we found that representatives of the Nocardiaceae family of the phylum Actinomycetota were tied to the areas where gas hydrates were found. The ability of aerobic and facultative anaerobic hydrocarbon-oxidizing microorganisms to anaerobically utilize hydrocarbons was determined with cultured methods. For the first time, this ability was revealed for the genera Stenotrophomonas , Psychrobacter , Micrococcus and Peribacillus . The wide distribution of this ability that we found in strains isolated from both study regions suggests its prominent role in the destruction of hydrocarbons in marine sediments. |
