Black Garlic and Thiosulfinate-Enriched Extracts as Adjuvants to Ceftriaxone Treatment in a Rat Peritonitis Model of Sepsis.

Autor: Redondo-Calvo FJ; Department of Anesthesiology and Critical Care Medicine, University General Hospital, 13004 Ciudad Real, Spain.; Translational Research Unit, University General Hospital and Research Institute of Castilla-La Mancha (IDISCAM), 13004 Ciudad Real, Spain.; Faculty of Medicine, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain., Bejarano-Ramírez N; Faculty of Medicine, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.; Department of Pediatrics, University General Hospital, 13004 Ciudad Real, Spain., Baladrón V; Department of Anesthesiology and Critical Care Medicine, University General Hospital, 13004 Ciudad Real, Spain., Montenegro O; Department of Anesthesiology and Critical Care Medicine, University General Hospital, 13004 Ciudad Real, Spain., Gómez LA; Department of Chemical Engineering, Institute of Chemical and Environmental Technology, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain., Velasco R; Department of Anesthesiology and Critical Care Medicine, University General Hospital, 13004 Ciudad Real, Spain., Villasanti N; Faculty of Medicine, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.; Department of Pathological Anatomy, University General Hospital, 13004 Ciudad Real, Spain., Illescas S; Faculty of Medicine, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.; Department of Microbiology, University General Hospital, 13004 Ciudad Real, Spain., Franco-Sereno MT; Department of Pharmacy, University General Hospital, 13004 Ciudad Real, Spain., Gracia I; Department of Chemical Engineering, Institute of Chemical and Environmental Technology, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain., Rodríguez JF; Department of Chemical Engineering, Institute of Chemical and Environmental Technology, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain., Muñoz-Rodríguez JR; Translational Research Unit, University General Hospital and Research Institute of Castilla-La Mancha (IDISCAM), 13004 Ciudad Real, Spain.; Faculty of Medicine, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain., Pérez-Ortiz JM; Translational Research Unit, University General Hospital and Research Institute of Castilla-La Mancha (IDISCAM), 13004 Ciudad Real, Spain.; Faculty of Medicine, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.
Jazyk: angličtina
Zdroj: Biomedicines [Biomedicines] 2022 Dec 01; Vol. 10 (12). Date of Electronic Publication: 2022 Dec 01.
DOI: 10.3390/biomedicines10123095
Abstrakt: To date, there have been no new drugs or adjuvants able to decrease both morbidity and mortality in the context of sepsis and septic shock. Our objective was to evaluate the use of thiosulfinate-enriched Allium sativum and black garlic extracts as adjuvants in the management of sepsis. An experimental in vivo study was carried out with male Sprague-Dawley ® rats. Animals were randomized in four treatment groups: antibiotic (ceftriaxone) treatment (group I), ceftriaxone plus thiosulfinate-enriched extract (TASE, group II), ceftriaxone plus thiosulfinate-enriched extract and black garlic extracts (TASE + BGE, group III), and ceftriaxone plus black garlic extract (BGE, group IV). All animals were housed and inoculated with 1 × 10 10 CFU/15 mL of intraperitoneal Escherichia coli ATCC 25922. Subsequently, they received a daily treatment according to each group for 7 days. Clinical, analytical, microbiological, and histopathological parameters were evaluated. Statistically significant clinical improvement was observed in rats receiving garlic extracts in weight (groups II and III), ocular secretions, and piloerection (group IV). Moreover, less liver edema, vacuolization, and inflammation were observed in groups receiving adjuvant support (groups II, III, and IV). When comparing interleukins 24 h after bacteria inoculum, we found statistically significant differences in TNF-alpha levels in groups receiving BGE (groups III and IV, p ≤ 0.05). Blood and peritoneal liquid cultures were also analyzed, and we detected a certain level of Enterococcus faecalis in peritoneal cultures from all treatment groups and less bacteria presence in blood cultures in rats receiving garlic extracts (groups II, III, and IV). In conclusion, TASE and BGE could be promising nutraceutical or medicinal agents as coadjuvants in the treatment of sepsis because of its effects in modulating the inflammatory response.
Databáze: MEDLINE