| Autor: |
Ganly I; Head and Neck Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA., Hao Y; Department of Pathology, NYU Grossman School of Medicine, New York, NY 10016, USA.; Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA., Rosenthal M; Head and Neck Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA., Wang H; Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.; Division of Infectious Diseases, Shenzhen Children's Hospital, Shenzhen 518026, China., Migliacci J; Head and Neck Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA., Huang B; Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.; Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China., Katabi N; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA., Brown S; Applied Bioinformatics Laboratories, NYU Grossman School of Medicine, New York, NY 10016, USA., Tang YW; Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.; Medical Affairs, Cepheid, Danaher Diagnostic Platform, Shanghai 200335, China., Pei Z; Department of Pathology, NYU Grossman School of Medicine, New York, NY 10016, USA.; Department of Medicine, NYU Grossman School of Medicine, New York, NY 10016, USA.; Pathology and Laboratory Medicine Service, Department of Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, USA., Yang L; Department of Pathology, NYU Grossman School of Medicine, New York, NY 10016, USA.; Department of Medicine, NYU Grossman School of Medicine, New York, NY 10016, USA. |
| Abstrakt: |
Objectives: Smoking is the commonest cause of oral cavity squamous cell carcinoma (OC-SCC), but the etiology of OC-SCC in nonsmokers is unknown. Our primary goal was to use metagenomic shotgun sequencing (MSS) to define the taxonomic composition and functional potential of oral metagenome in nonsmokers with OC-SCC. Methods: We conducted a case-control study with 42 OC-SCC case and 45 control nonsmokers. MSS was performed on DNA extracted from mouthwash samples. Taxonomic analysis and pathway analysis were done using MetaPhlAn2 and HUMAnN2, respectively. Statistical difference was determined using the Mann-Whitney test controlling false discovery rate. Results: There was no significant difference in age, sex, race, or alcohol consumption between OC-SCC and control patients. There was a significant difference in beta diversity between OC-SCC and controls. At the phylum level, Bacteroidetes and Synergistetes were overly represented in OC-SCC while Actinobacteria and Firmicutes were overly represented in controls. At the genus level, Fusobacterium was overly represented in OC-SCC compared with controls, while Corynebacterium , Streptococcus , Actinomyces , Cryptobacterium , and Selenomonas were overly represented in controls. Bacterial pathway analysis identified overrepresentation in OC-SCC of pathways related to metabolism of flavin, biotin, thiamin, heme, sugars, fatty acids, peptidoglycans, and tRNA and overrepresentation of nucleotides and essential amino acids in controls. Conclusions : The oral microbiome in nonsmoker patients with OC-SCC is significantly different from that of nonsmoker control patients in taxonomic compositions and functional potentials. Our study's MSS findings matched with previous 16S-based methods in taxonomic differentiation but varied greatly in functional differentiation of microbiomes in OC-SCC and controls. |
