| Autor: |
Wehner TA; Merck Sharp and Dohme Research Laboratories, Rahway, NJ 07065., Wood JS Jr, Walker R, Downing GV, Vandenheuvel WJ |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1987 Jul 24; Vol. 399, pp. 251-8. |
| Abstrakt: |
A confirmatory assay for residues of the anthelmintic agent clorsulon [4-amino-6-(trichloroethenyl)-1,3-benzenedisulfonamide] in cattle kidney tissue has been developed. The assay involves isolation of a drug-containing fraction by solvent extraction, methylation of the analyte, and fused-silica capillary column gas chromatography-negative-ion chemical-ionization mass spectrometry of the pentamethyl derivative of clorsulon. The intensities of four negative ions [m/z 406 and 408 (trichloro species) and m/z 413 and 415 (dichloro species)] are monitored. Confirmation of the presence of drug in an analyte requires that all four ions appear at the appropriate retention time with their intensity ratios within 10-15% of those arising from analysis of the reference standard, methylated clorsulon; the lower limit of detection is 3 ppb. Quantification of the drug is based on the intensity of the m/z 406 ion. Identification and quantification of residues by the gas chromatographic-mass spectrometric assay gave results in good agreement with those obtained with an electron-capture gas chromatographic assay. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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