Microbiological Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy.

Autor:	Braga GPA; Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil., Caiaffa KS; Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil., Pereira JA; Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil., Santos VRD; Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil., Souza ACA; Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil., Ribeiro LDS; Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, São Paulo 13565-905, Brazil., Camargo ER; Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, São Paulo 13565-905, Brazil., Prakki A; Department of Clinical Sciences-Restorative Dentistry, Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada., Duque C; Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil.; Department of Clinical Sciences-Restorative Dentistry, Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada.
Jazyk:	angličtina
Zdroj:	Journal of functional biomaterials [J Funct Biomater] 2022 Dec 17; Vol. 13 (4). Date of Electronic Publication: 2022 Dec 17.
DOI:	10.3390/jfb13040305
Abstrakt:	This study aimed to evaluate the cytotoxicity and microbiological properties of poly (N-vinylcaprolactam)-PNVCL hydrogels containing flavonoids as intracanal medication for endodontic therapy. Antimicrobial activity of ampelopsin (AMP), isoquercitrin and rutin was determined against Enterococcus faecalis , Actinomyces israelii , Lactobacillus casei , Streptococcus mutans , and Fusobacterium nucleatum by the microdilution method. After synthesis and characterization by rheology, PNVCL hydrogels were loaded with AMP and controls calcium hydroxide (CH) and chlorhexidine (CHX), and determined the compounds release profile. PNVCL+AMP, PNVCL+CH, PNVCL+CHX were evaluated on multi-species biofilms and analyzed by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM). Cytotoxicity was determined after fibroblasts exposure to serial dilutions of AMP and PNVCL hydrogel extracts. AMP was effective against all of the bacteria tested, especially against S. mutans , A. israelli and F. nucleatum . SEM and CLSM analysis showed that PNVCL + AMP caused a significant decrease and disorganization of multi-species biofilms and reduction of intracanal viable cells, superior to the other groups. AMP affected fibroblast viability at concentrations above 0.125 mg/mL, and extracts of PNVCL+AMP showed low cytotoxicity. In conclusion, PNVCL containing AMP demonstrated cytocompatibility and potent effect against multi-species biofilms and could be potential intracanal medication for endodontic purposes. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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