Solid-state NMR - a complementary technique for protein framework characterization.

Autor: Cerofolini L; Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, Sesto Fiorentino 50019, Italy. fragai@cerm.unifi.it.; Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine (CIRMMP), Via L. Sacconi 6, Sesto Fiorentino 50019, Italy., Ramberg KO; School of Biological and Chemical Sciences, University of Galway, University Road, Galway H91 TK33, Ireland. peter.crowley@nuigalway.ie., Padilla LC; Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, Sesto Fiorentino 50019, Italy. fragai@cerm.unifi.it.; Department of Chemistry 'Ugo Schiff', University of Florence, Via della Lastruccia 3, Sesto Fiorentino 50019, Italy., Antonik P; School of Biological and Chemical Sciences, University of Galway, University Road, Galway H91 TK33, Ireland. peter.crowley@nuigalway.ie., Ravera E; Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, Sesto Fiorentino 50019, Italy. fragai@cerm.unifi.it.; Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine (CIRMMP), Via L. Sacconi 6, Sesto Fiorentino 50019, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Via della Lastruccia 3, Sesto Fiorentino 50019, Italy., Luchinat C; Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, Sesto Fiorentino 50019, Italy. fragai@cerm.unifi.it.; Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine (CIRMMP), Via L. Sacconi 6, Sesto Fiorentino 50019, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Via della Lastruccia 3, Sesto Fiorentino 50019, Italy., Fragai M; Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, Sesto Fiorentino 50019, Italy. fragai@cerm.unifi.it.; Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine (CIRMMP), Via L. Sacconi 6, Sesto Fiorentino 50019, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Via della Lastruccia 3, Sesto Fiorentino 50019, Italy., Crowley PB; School of Biological and Chemical Sciences, University of Galway, University Road, Galway H91 TK33, Ireland. peter.crowley@nuigalway.ie.
Jazyk: angličtina
Zdroj: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2023 Jan 17; Vol. 59 (6), pp. 776-779. Date of Electronic Publication: 2023 Jan 17.
DOI: 10.1039/d2cc05725e
Abstrakt: Protein frameworks are an emerging class of biomaterial with medical and technological applications. Frameworks are studied mainly by X-ray diffraction or scattering techniques. Complementary strategies are required. Here, we report solid-state NMR analyses of a microcrystalline protein-macrocycle framework and the rehydrated freeze-dried protein. This methodology may aid the characterization of low-crystallinity frameworks.
Databáze: MEDLINE
Externí odkaz: