| Autor: |
Silvestri A; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain. mprato@cicbiomagune.es., Zayas-Arrabal J; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain. mprato@cicbiomagune.es., Vera-Hidalgo M; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain. mprato@cicbiomagune.es., Di Silvio D; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain. mprato@cicbiomagune.es., Wetzl C; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain. mprato@cicbiomagune.es.; University of the Basque Country UPV-EHU, 20018 Donostia-San Sebastián, Spain., Martinez-Moro M; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain. mprato@cicbiomagune.es., Zurutuza A; Graphenea Semiconductor SLU., Paseo Mikeletegi 83, 20009 San Sebastián, Spain., Torres E; Graphenea Semiconductor SLU., Paseo Mikeletegi 83, 20009 San Sebastián, Spain., Centeno A; Graphenea Semiconductor SLU., Paseo Mikeletegi 83, 20009 San Sebastián, Spain., Maestre A; Graphenea Semiconductor SLU., Paseo Mikeletegi 83, 20009 San Sebastián, Spain., Gómez JM; Graphenea Semiconductor SLU., Paseo Mikeletegi 83, 20009 San Sebastián, Spain., Arrastua M; Graphenea Semiconductor SLU., Paseo Mikeletegi 83, 20009 San Sebastián, Spain., Elicegui M; Graphenea Semiconductor SLU., Paseo Mikeletegi 83, 20009 San Sebastián, Spain., Ontoso N; Graphenea Semiconductor SLU., Paseo Mikeletegi 83, 20009 San Sebastián, Spain., Prato M; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain. mprato@cicbiomagune.es.; Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via L. Giorgieri 1, 3412 7 Trieste, Italy.; Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain., Coluzza I; BCMaterials, Basque Center for Materials, Applications and Nanostructures, Bld. Martina Casiano, UPV/EHU Science Park, Barrio Sarriena s/n, 48940 Leioa, Spain. ivan.coluzza@bcmaterials.net.; Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain., Criado A; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain. mprato@cicbiomagune.es.; Universidade da Coruña, CICA - Centro Interdisciplinar de Química e Bioloxía, Rúa as Carballeiras, 15071 A Coruña, Spain. a.criado@udc.es. |
| Abstrakt: |
COVID-19, caused by the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), originated a global health crisis, causing over 2 million casualties and altering human daily life all over the world. This pandemic emergency revealed the limitations of current diagnostic tests, highlighting the urgency to develop faster, more precise and sensitive sensors. Graphene field effect transistors (GFET) are analytical platforms that enclose all these requirements. However, the design of a sensitive and robust GFET is not a straightforward objective. In this work, we report a GFET array biosensor for the detection of SARS-CoV-2 spike protein using the human membrane protein involved in the virus internalisation: angiotensin-converting enzyme 2 (ACE2). By finely controlling the graphene functionalisation, by tuning the Debye length, and by deeply characterising the ACE2-spike protein interactions, we have been able to detect the target protein with an extremely low limit of detection (2.94 aM). This work set the basis for a new class of analytical platforms, based on human membrane proteins, with the potential to detect a broad variety of pathogens, even before their isolation, being a powerful tool in the fight against future pandemics. |
