Omicron infection increases IgG binding to spike protein of predecessor variants.

Autor: Mahalingam G; Centre for Stem Cell Research (CSCR) (a unit of inStem, Bengaluru), Christian Medical College, Vellore, Tamil Nadu, India., Periyasami Y; Centre for Stem Cell Research (CSCR) (a unit of inStem, Bengaluru), Christian Medical College, Vellore, Tamil Nadu, India., Arjunan P; Centre for Stem Cell Research (CSCR) (a unit of inStem, Bengaluru), Christian Medical College, Vellore, Tamil Nadu, India., Subaschandrabose RK; Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India., Mathivanan TV; Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India., Mathew RS; Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India., Devi RKT; Department of Biotechnology, SRM Institute of Science and Technology, Tamil Nadu, India., Premkumar PS; Department of Biostatistics, Christian Medical College, Vellore, Tamil Nadu, India., Muliyil J; Christian Medical College, Vellore, Tamil Nadu, India., Srivastava A; Centre for Stem Cell Research (CSCR) (a unit of inStem, Bengaluru), Christian Medical College, Vellore, Tamil Nadu, India., Moorthy M; Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India., Marepally S; Centre for Stem Cell Research (CSCR) (a unit of inStem, Bengaluru), Christian Medical College, Vellore, Tamil Nadu, India.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2023 Feb; Vol. 95 (2), pp. e28419.
DOI: 10.1002/jmv.28419
Abstrakt: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission in India in 2020-2022 was driven predominantly by Wild (Wuhan-Hu-1 and D614G), Delta, and Omicron variants. The aim of this study was to examine the effect of infections on the humoral immune response and cross-reactivity to spike proteins of Wuhan-Hu-1, Delta, C.1.2., and Omicron. Residual archival sera (N = 81) received between January 2020 and March 2022 were included. Infection status was inferred by a positive SARS-CoV-2 RT-PCR and/or serology (anti-N and anti-S antibodies) and sequencing of contemporaneous samples (N = 18) to infer lineage. We estimated the levels and cross-reactivity of infection-induced sera including Wild, Delta, Omicron as well as vaccine breakthrough infections (Delta and Omicron). We found an approximately two-fold increase in spike-specific IgG antibody binding in post-Omicron infection compared with the pre-Omicron period, whilst the change in pre- and post-Delta infections were similar. Further investigation of Omicron-specific humoral responses revealed primary Omicron infection as an inducer of cross-reactive antibodies against predecessor variants, in spite of the weaker degree of humoral response compared to Wuhan-Hu-1 and Delta infection. Intriguingly, Omicron vaccine-breakthrough infections when compared with primary infections, exhibited increased humoral responses against RBD (7.7-fold) and Trimeric S (Trimeric form of spike protein) (34.6-fold) in addition to increased binding of IgGs towards previously circulating variants (4.2 - 6.5-fold). Despite Delta breakthrough infections showing a higher level of humoral response against RBD (2.9-fold) and Trimeric S (5.7-fold) compared to primary Delta sera, a demonstrably reduced binding (36%-49%) was observed to Omicron spike protein. Omicron vaccine breakthrough infection results in increased intensity of humoral response and wider breadth of IgG binding to spike proteins of antigenically-distinct, predecessor variants.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: