Genetic encoding of isobutyryl-, isovaleryl-, and β-hydroxybutryl-lysine in E. coli .
| Autor: | Christopher JA; Department of Chemistry, Virginia Commonwealth University Richmond VA 23284 USA tacropp@vcu.edu., Galbada Liyanage SA; Department of Chemistry, Virginia Commonwealth University Richmond VA 23284 USA tacropp@vcu.edu., Nicholson EM; Department of Chemistry, Virginia Commonwealth University Richmond VA 23284 USA tacropp@vcu.edu., Kinney WD; Department of Chemistry, Virginia Commonwealth University Richmond VA 23284 USA tacropp@vcu.edu., Cropp TA; Department of Chemistry, Virginia Commonwealth University Richmond VA 23284 USA tacropp@vcu.edu. |
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| Jazyk: | angličtina |
| Zdroj: | RSC advances [RSC Adv] 2022 Nov 29; Vol. 12 (53), pp. 34142-34144. Date of Electronic Publication: 2022 Nov 29 (Print Publication: 2022). |
| DOI: | 10.1039/d2ra04898a |
| Abstrakt: | Here we report the synthesis and genetic encoding of the lysine post translational modifications, β-hydroxybutyryl-lysine, isobutyryl-lysine and isovaleryl-lysine. The ability to obtain a homogenous protein samples with site-specific incorporation of these acylated lysine residues can serve as a powerful tool to study the biological role of lysine post translational modifications. Competing Interests: The authors indicate there are no conflicts to declare. (This journal is © The Royal Society of Chemistry.) |
| Databáze: | MEDLINE |
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