Lentinus squarrosulus Mont. Mushroom: Molecular Identification, In vitro Anti-Diabetic, Anti-Obesity, and Cytotoxicity Assessment.
| Autor: | Abiodun OO; University of Ibadan, Faculty of Basic Medical Sciences, Department of Pharmacology and Therapeutics, Ibadan, Nigeria.; University of Ibadan, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Ibadan, Nigeria., Alege AM; University of Ibadan, Faculty of Basic Medical Sciences, Department of Pharmacology and Therapeutics, Ibadan, Nigeria., Ezurike PU; Michael Okpara University of Agriculture, College of Natural Sciences, Department of Biochemistry, Umudike, Nigeria., Nkumah A; University of Ibadan, Faculty of Pharmacy, Department of Pharmacognosy, Ibadan, Nigeria., Adelowo O; University of Ibadan, Faculty of Basic Medical Sciences, Department of Pharmacology and Therapeutics, Ibadan, Nigeria., Oke TA; University of Ibadan, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Ibadan, Nigeria. |
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| Jazyk: | angličtina |
| Zdroj: | Turkish journal of pharmaceutical sciences [Turk J Pharm Sci] 2022 Dec 21; Vol. 19 (6), pp. 642-648. |
| DOI: | 10.4274/tjps.galenos.2021.72798 |
| Abstrakt: | Objectives: Mushrooms are fungi with nutritional and health benefits. Lentinus squarrosulus Mont., an edible fungus, has traditional usage and relevance in local therapy for managing metabolic diseases. In that view, this study aimed to evaluate the in vitro anti-obesity, anti-diabetic, and cytotoxic potential of the chloroform/methanol extract (CME) and aqueous extract (AE) of the mushroom. Materials and Methods: L. squarrosulus was identified using molecular biology tools. The CME and AE were obtained sequentially and, then, subjected to α-amylase, α-glucosidase, and lipase inhibitory enzyme assays as well as total phenolic content (TPC) and flavonoid content (TFC) estimations. The cytotoxic potential of extract fractions of L. squarrosulus was assessed using the brine shrimp lethality assay. Results: The molecular identification of the mushroom displayed that the internal transcribed spacer sequence was an equivalent match to that of L. squarrosulus with a high percentage similarity, and thus assigned a unique accession number (KT120043.1). The CME of L. squarrosulus had higher TPC, TFC, and α-glucosidase inhibitory activity than AE. Furthermore, AE of the mushroom showed a higher lipase inhibitory potential with an IC Conclusion: This study reveals that CME of L. squarrosulus , rich in polyphenols and flavonoids, possesses considerable α-glucosidase and lipase inhibitory activities. Competing Interests: Conflict of Interest: No conflict of interest was declared by the authors. (©Turk J Pharm Sci, Published by Galenos Publishing House.) |
| Databáze: | MEDLINE |
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