A modified hydrogel production protocol to decrease cellular content.

Autor:	Braga GCD; Bachelor. Universidade de São Paulo - Discipline of Plastic Surgery, Microsurgery and Plastic Surgery Laboratory - School of Medicine - São Paulo (SP), Brazil., Camargo CP; PhD. Universidade de São Paulo - Discipline of Plastic Surgery, Microsurgery and Plastic Surgery Laboratory - School of Medicine - São Paulo (SP), Brazil., Harmsen MC; PhD. Associate professor. University Medical Center Groningen - Laboratory for Cardiovascular Regenerative Medicine - Department of Pathology and Medical Biology - Hanzeplein 1, Netherlands., Correia AT; PhD. Universidade de São Paulo - Department of Cardiopneumology - Thoracic Surgery Research Laboratory - Heart Institute of School of Medicine - São Paulo (SP), Brazil., Souza S; Bachelor. Universidade de São Paulo - Department of Cardiopneumology - Cardiovascular Surgery and Circulatory Physiopathology Laboratory - School of Medicine - São Paulo (SP), Brazil., Seelaender M; PhD. Associate professor. Universidade de São Paulo - Department of Clinical Surgery - School of Medicine - São Paulo (SP), Brazil., Nunes VA; PhD. Associate professor. Universidade de São Paulo - Department of Biotechnology - School of Arts, Sciences and Humanities - São Paulo (SP), Brazil., Santos JFD; PhD. Universidade de São Paulo - Department of Biotechnology - School of Arts, Sciences and Humanities - São Paulo (SP), Brazil., Neri EA; PhD. Universidade de São Paulo - Laboratory of Genetics and Molecular Cardiology - Heart Institute - School of Medicine - São Paulo (SP), Brazil., Valadão IC; PhD. Universidade de São Paulo - Laboratory of Genetics and Molecular Cardiology - Heart Institute - School of Medicine - São Paulo (SP), Brazil., Moreira LFP; PhD. Associate professor. Universidade de São Paulo - Department of Cardiopneumology, Cardiovascular Surgery and Circulatory Physiopathology Laboratory - School of Medicine - São Paulo (SP), Brazil., Gemperli R; PhD. Full professor. Universidade de São Paulo - Discipline of Plastic Surgery, Microsurgery and Plastic Surgery Laboratory - School of Medicine - São Paulo (SP) Brazil.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2022 Dec 19; Vol. 37 (10), pp. e371005. Date of Electronic Publication: 2022 Dec 19 (Print Publication: 2022).
DOI:	10.1590/acb371005
Abstrakt:	Purpose: To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix. Methods: We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation. Results: After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days. Conclusions: The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu