Imaging of Heart Type Fatty Acid Binding Protein Under Acute Reperfusion Ischemia Using Radio-labeled Antibody in Rat Heart Model.

Autor: Fukushima K; Department of Nuclear Medicine, Saitama Medical University International Medical Center, Japan.; Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women's Medical University, Japan., Momose M; Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women's Medical University, Japan., Kanaya K; Department of Radiological Service, Tokyo Women's Medical University, Japan., Kaimoto Y; Department of Radiological Service, Tokyo Women's Medical University, Japan., Higuchi T; Department of Nuclear Medicine, University of Wuerzburg, Comprehensive Heart Failure Center, University of Wuerzburg, Germany., Yamamoto A; Department of Cardiology, Tokyo Women's Medical University, Japan., Nakao R; Department of Cardiology, Tokyo Women's Medical University, Japan., Matsuo Y; Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women's Medical University, Japan., Nagao M; Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women's Medical University, Japan., Kuji I; Department of Nuclear Medicine, Saitama Medical University International Medical Center, Japan., Abe K; Department of Radiology, Tokyo Medical University, Japan.
Jazyk: angličtina
Zdroj: Annals of nuclear cardiology [Ann Nucl Cardiol] 2022; Vol. 8 (1), pp. 14-20. Date of Electronic Publication: 2022 Aug 31.
DOI: 10.17996/anc.21-00146
Abstrakt: Purpose : Heart-type fatty acid binding protein (H-FABP) is primary transporter of free fatty acid and plays an important role in myocardial metabolism, which is characterized by high specificity and rapid appearance under ischemic condition. The objective of this study was to clarify the usefulness of imaging study of targeting H-FABP appearance using radio-labeled antibody, and correlation with myocardial fatty acid metabolism and perfusion in acute reperfusion ischemia. Method : Wistar rats were allotted to sham-operated control group (sham; n=4), ischemia non-reperfused group (IG; n=5), and ischemia-reperfusion group (RG; n=5). Ligation of left coronary artery (LCA) was performed for IG and RG. 20 min of ischemia was followed by 60min of reperfusion for RG. 125 I labeled anti H-FABP antibody (anti H-FABP), BMIPP and 99m Tc-sestamibi (MIBI) was injected intravenously. Multi-tracer digital autoradiogram was performed using µ-imager ® . The ratio of radioactivity in LCA related (culprit) area to the inferior (remote) area (target uptake ratio=TUR) was generated. Results : In sham group, no visually detectable accumulation was observed for the anti H-FABP image, and TUR MIBI and TUR BMIPP were equivalent to 1. In IG, TUR MIBI and TUR BMIPP were remarkably low (0.12±0.01, 0.24±0.07). In RG, TUR MIBI was significantly lower (0.20±0.03, p<0.05 vs. other groups). However, TUR BMIPP was significantly higher (2.78±1.28, p<0.05) compared to the sham and IG, whereas anti H-FABP showed markedly higher ratio in the reperfused area compared to the sham and IG (3.43±0.73 vs. 0.31±0.13 and 1.09±0.07 for IG and sham; p<0.05, and <0.01, respectively). Conclusion : Anti H-FABP accumulated specifically in reperfused area under acute ischemia, and it accorded to the area where fatty acid metabolism was activated. This study has shown the future potential for clinical application in vivo imaging of acute coronary syndrome.
Competing Interests: None.
(2022, The Japanese Society of Nuclear Cardiology.)
Databáze: MEDLINE
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