Blockage of Nrf2 and autophagy by L-selenocystine induces selective death in Nrf2-addicted colorectal cancer cells through p62-Keap-1-Nrf2 axis.

Autor: Hsu WL; Department of Life Sciences, National Central University, Taoyuan, Taiwan., Wang CM; Department of Life Sciences, National Central University, Taoyuan, Taiwan., Yao CL; Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan., Chen SC; Department of Life Sciences, National Central University, Taoyuan, Taiwan., Nien CY; Department of Life Sciences, National Central University, Taoyuan, Taiwan., Sun YH; Department of Life Sciences, National Central University, Taoyuan, Taiwan., Tseng TY; Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan., Luo YH; Department of Life Sciences, National Central University, Taoyuan, Taiwan. yhLuo@g.ncu.edu.tw.
Jazyk: angličtina
Zdroj: Cell death & disease [Cell Death Dis] 2022 Dec 20; Vol. 13 (12), pp. 1060. Date of Electronic Publication: 2022 Dec 20.
DOI: 10.1038/s41419-022-05512-2
Abstrakt: Persistent Nrf2 activation is typically noted in many cancers, including colorectal cancer (CRC), aiding cancer cells in overcoming growth stress and promoting cancer progression. Sustained Nrf2 activation, which is beneficial for cancer cells, is called "Nrf2 addiction"; it is closely associated with malignancy and poor prognosis in patients with cancer. However, Nrf2 inhibitors may have adverse effects on normal cells. Here, we found that the selenocompound L-selenocystine (SeC) is selectively cytotoxic in the Nrf2-addicted CRC cell line WiDr cells, but not in non-Nrf2-addicted mesenchymal stem cells (MSCs) and normal human colon cells. Another CRC cell line, C 2 BBe 1 , which harbored lower levels of Nrf2 and its downstream proteins were less sensitive to SeC, compared with the WiDr cells. We further demonstrated that SeC inhibited Nrf2 and autophagy activation in the CRC cells. Antioxidant GSH pretreatment partially rescued the CRC cells from SeC-induced cytotoxicity and Nrf2 and autophagy pathway inhibition. By contrast, SeC activated Nrf2 and autophagy pathway in non-Nrf2-addicted MSCs. Transfecting WiDr cells with Nrf2-targeting siRNA decreased persistent Nrf2 activation and alleviated SeC cytotoxicity. In KEAP1-knockdown C 2 BBe 1 cells, Nrf2 pathway activation increased SeC sensitivity and cytotoxicity. In conclusion, SeC selectively attacks cancer cells with constitutively activated Nrf2 by reducing Nrf2 and autophagy pathway protein expression through the P62-Nrf2-antioxidant response element axis and eventually trigger cell death.
(© 2022. The Author(s).)
Databáze: MEDLINE
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