In Vitro Comparison of Local Nasal Vaccine Delivery and Correlation with Device Spray Performance.

Autor: Li L; Vaccine CMC Development & Supply, Sanofi, Toronto, Room 121, Building 95, Sanofi, 1755 Steeles Avenue West, Toronto, ON, M2R 3T4, USA. lillian.li@Sanofi.com., Wilkins JV Jr; Department of Mechanical and Nuclear Engineering, Virginia Commonwealth University, Room 1083, 800 E. Leigh St, Richmond, VA, 23298, USA., Esmaeili AR; Department of Mechanical and Nuclear Engineering, Virginia Commonwealth University, Room 1083, 800 E. Leigh St, Richmond, VA, 23298, USA., Rahman N; Vaccine CMC Development & Supply, Sanofi, Toronto, Room 121, Building 95, Sanofi, 1755 Steeles Avenue West, Toronto, ON, M2R 3T4, USA., Golshahi L; Department of Mechanical and Nuclear Engineering, Virginia Commonwealth University, Room 1083, 800 E. Leigh St, Richmond, VA, 23298, USA. lgolshahi@vcu.edu.
Jazyk: angličtina
Zdroj: Pharmaceutical research [Pharm Res] 2023 Feb; Vol. 40 (2), pp. 537-550. Date of Electronic Publication: 2022 Dec 19.
DOI: 10.1007/s11095-022-03452-2
Abstrakt: Purpose: This study is the first vaccine candidate in vitro investigation with a focus on finding a correlation between the spray characteristics and the delivery efficiency of the local deposition in the nasal airways of infants under 24 months using various intranasal devices.
Methods: In vitro tests were developed to measure the spray characteristics of four intranasal delivery devices and how they regionally deliver a candidate vaccine formulation matrix in five nasal airway replicas (3 to 24 months). The correlation between the spray performance, geometric parameters, and delivery efficiency were assessed.
Results: All four devices performed consistently in terms of spray characteristics and were capable of delivering a high percentage of the candidate vaccine to the target areas, with a minimum delivery efficiency of 80%. Moreover, the delivery efficiency was affected by either the spray droplet size distribution or the distance between the nozzle tip and the internal nasal valve. Correlations between the spray performance and the in vitro local dose deposition were established.
Conclusion: The infant nasal model tests can be complementary to device spray performance evaluation. In the absence of in vivo correlations, they can also facilitate the process of new product development by estimating delivery a priori.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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