Whole transcriptome expression profiles in kidney samples from rats with hyperuricaemic nephropathy.

Autor: Li N; College of Pharmacy, Southwest Minzu University, Chengdu, China., Amatjan M; College of Pharmacy, Southwest Minzu University, Chengdu, China., He P; College of Pharmacy, Southwest Minzu University, Chengdu, China., Wu M; College of Pharmacy, Southwest Minzu University, Chengdu, China., Yan H; College of Pharmacy, Southwest Minzu University, Chengdu, China., Shao X; College of Pharmacy, Southwest Minzu University, Chengdu, China.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Dec 19; Vol. 17 (12), pp. e0276591. Date of Electronic Publication: 2022 Dec 19 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0276591
Abstrakt: Hyperuricaemic nephropathy (HN) is a common clinical complication of hyperuricaemia (HUA) and poses a huge threat to human health. Hence, we aimed to prospectively investigate the dysregulated genes, pathways and networks involved in HN by performing whole transcriptome sequencing using RNA sequencing. Six kidney samples from HN group (n = 3) and a control group (n = 3) were obtained to conduct RNA sequencing. To disclose the relevant signalling pathways, we conducted the analysis of differentially expressed genes (DEGs), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. A competitive endogenous RNA (ceRNA) network was established to reveal the interactions between lncRNAs, circRNAs, mRNAs and miRNAs and investigate the potential mechanisms of HN. Ultimately, 2250 mRNAs, 306 lncRNAs, 5 circRNAs, and 70 miRNAs were determined to be significantly differentially expressed in the HN group relative to the control group. We further authenticated 8 differentially expressed (DE)-ncRNAs by quantitative real-time polymerase chain reaction, and these findings were in accordance with the sequencing results. The analysis results evidently showed that these DE-ncRNAs were significantly enriched in pathways related to inflammatory reaction. In conclusion, HUA may generate abnormal gene expression changes and regulate signalling pathways in kidney samples. Potentially related genes and pathways involved in HN were identified.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2022 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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