Protective Effects of Kefir Against Unpredictable Chronic Stress Alterations in Mice Central Nervous System, Heart, and Kidney.

Autor: Silva AO; Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., Ribeiro JM; Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., Patrocínio TB; Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., Amorim GE; Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., Pereira-Júnior AA; Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., Ângelo ML; Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., de Araújo Paula FB; Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., de Mello Silva Oliveira N; José Do Rosário Velano University (UNIFENAS), Alfenas, MG, Brazil., Ruginsk SG; Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., Antunes-Rodrigues J; University of São Paulo, Ribeirão Preto, São Paulo, Brazil., Elias LLK; University of São Paulo, Ribeirão Preto, São Paulo, Brazil., Dias MVS; Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., Torres LH; Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, Brazil., Ceron CS; Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Diogo de Vasconcelos, 122, 35400-000l, Ouro Preto, MG, Brazil. carla.ceron@ufop.edu.br.
Jazyk: angličtina
Zdroj: Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2023 Apr; Vol. 15 (2), pp. 411-423. Date of Electronic Publication: 2022 Dec 19.
DOI: 10.1007/s12602-022-10031-9
Abstrakt: Kefir is a probiotic mixture with anxiolytic and antioxidant properties. Chronic stress can lead to anxiety disorders and increase oxidative damage in organs such as the heart and kidney. In this study, we examined whether kefir ameliorates the anxiety-like behavior of mice submitted to chronic unpredictable stress (CUS) by modulating brain-derived neurotrophic factor (BDNF) and corticosterone levels and whether kefir modifies the oxidative parameters in the heart and kidney of mice. Male Swiss mice received kefir (0.3 mL/100 g/day) or milk for 30 days (gavage). On the 10th day, the mice were submitted to CUS. Behavioral analysis was performed using the elevated plus maze and forced swimming tests. BDNF levels were analyzed in brain tissues. Heart and kidney superoxide dismutase (SOD), catalase, glutathione (GSH), thiobarbituric acid reactive substances (TBARS), 3-nitrotyrosine, metalloproteinase-2 (MMP-2), and plasma corticosterone were evaluated. Kefir reverted the CUS-induced decrease in the time spent in the open arms, the increase in grooming frequency, and decrease in the head dipping frequency, but not the reduced immobility time. CUS decreased the cerebellum BDNF levels and increased corticosterone levels, which were restored by Kefir. Neither catalase and SOD activities nor GSH, TBARS, 3-nitrotyrosine, and MMP-2 were modified by CUS in the heart. In the kidney, CUS increased 3-nitrotyrosine and MMP-2. Kefir increased the antioxidant defense in the heart and kidney of control and CUS mice. These results suggest that kefir ameliorated CUS-induced anxiety-like behavior by modulating brain BDNF and corticosterone levels. Kefir also increased the antioxidant defense of mice heart and kidney.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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